Re: [Histonet] Ventana Ultra vs. Leica Bond Max
Hello, We had Leica Bond Max instruments (2 Bonds) for about 5 years and made the move over the Ventana Ultras (3 Ultras) a couple years ago. We made the move initially for the speed and menu benefits versus the Leica, (Ultra is about 2x faster) but also saw a quality improvement once we tweaked the protocols a bit. I don't know about the difference between the XT and the Ultra because we never had the XT, but from the Leica to the Ultra the biggest improvement was speed. We also saw dirty H. Pylori staining at first with both Leica and Ventana but have cleaned it up dramatically with some processing and fixation changes. I don't know if the instrument has much to do with the staining quality as the changes in our process because the staining was improved using the same Ultra instrument. If you like I would be willing stain your tissue on our instruments to see if you do in fact have a bad instrument??? I can also share with you our protocols to see if that helps your staining issues. Thanks, Curt -Original Message- From: Gareth Davis via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, September 08, 2016 12:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Ultra vs. Leica Bond Max I was interested in anyone's opinion on the new Ventana Benchmark Ultra and Leica's Bond Max. I currently use the Ventana Benchmark XT. Ventana wants us to upgrade to the Ultra. Because our contract will be up in a year, my manager and lab owners want me to check out other options. Hoping to get a better deal and a better instrument. The Benchmark XT slide heaters keep going out and the H. pylori is awful, Ventana swears it's the instruments fault. Thanks, -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Ultra vs. Leica Bond Max
I was interested in anyone's opinion on the new Ventana Benchmark Ultra and Leica's Bond Max. I currently use the Ventana Benchmark XT. Ventana wants us to upgrade to the Ultra. Because our contract will be up in a year, my manager and lab owners want me to check out other options. Hoping to get a better deal and a better instrument. The Benchmark XT slide heaters keep going out and the H. pylori is awful, Ventana swears it's the instruments fault. Thanks, -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Ultra CD10
We are in the last stages of contract negotiations for purchasing two Ventana Benchmark Ultras. During this time period we have been optimizing our current inventory of approximately 110 antibodies and validating before our current instrumentation is removed within the next two weeks. My Ventana application specialist is unable to successfully optimize CD10 (Ventana clone SP67) on the Ventana Benchmark Ultra. He had me send slides to their applications/troubleshooting lab but they told us they won't start working on it until next week and then it would take about a week for them to try and optimize it. We are in an urgent rush to get this antibody optimized and validated within the next two weeks since it is heavily requested by our Hemepaths. Would anyone be willing to share their Ultra protocols with me? Has anyone had similar experiences with Ventana being unsuccessful and having to send their slides to their applications lab for work up? Thank you for your help. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC one...@wvuhealthcare.commailto:one...@wvuhealthcare.com Histology Supervisor, Technical Specialist Lab: 304 - 293 - 6014 Office: 304 - 293 - 7629 - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] VENTANA ULTRA ER,PR,HER2
The Her2 CB11 clone of Ventana is FDA approved. In the package insert of the Her2 4B5 clone is stated, that the antibody was compared to CB11 and shows same or better performance. The CB11 clone was exchanged through 4B5. On the NordiQC website Ventana 4B5 and Dako Herceptest are called FDA approved. http://www.nordiqc.org/Run-30-B10/Assessment/assessment-B10-HER2.htm So in my opinion the company wouldn't exchange its antibody, if it wouldn't be approved now or soon. Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Patsy Ruegg Gesendet: Samstag, 02. Juli 2011 18:09 An: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] VENTANA ULTRA ER,PR,HER2 I thought Ventana and Dako both had FDA approved Her2, I know Dako has Hercept Test. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Tuesday, June 28, 2011 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VENTANA ULTRA ER,PR,HER2 We are investigating getting the Ventana Ultra. I discovered that the ER, PR, HER2 are not yet FDA approved. If you are using the Ventana Ultra how are you doing the ER, PR, HER2? Do you use the Benchmark XT? Is anyone using the INFORM HER2 Dual ISH DNA Probe Cocktail Assay? ANTOINETTE CRILL, E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] VENTANA ULTRA ER,PR,HER2
I think Barbara was referring to the approval for the ER, PR and Her2 antibodies being for use with the Benchmark XT stainers and that they aren't yet approved for use on the ULTRA stainers using newer formulated bulk reagents. It's funny because they both use the same detection kits and antibodies. The formulation for the bulk reagents is different and it is a new instrument, so I think that's why they need another approval from the FDA. The antibodies were already approved for use with the Benchmark XT stainers. We aren't running Ventana's new Dual ISH Her2 probe kit but we will be looking at it. It does seem to have some perks from the lab's point of view. The instrument does the entire ISH procedure/stain. No more having to mix the reagents for the pretreatment as with Abbott's Pathvysion Kit, repeated pipetting, 22 x 22 coverslips or rubber cement. It's even worse if you don't have a VP2000 pretreatment unit, then you're stuck doing FISH pretreatment by hand with coplin jars in waterbaths. You don't need a fluorescent scope to see the slide with Ventana's new kit and it would be easy for the pathologist to make sure the correct area is scored for Her2 amplification (as can be a problem with the pathologist circling the area of interest on an HE and the tech matching it up to another unstained serial section with Her2 FISH). I know a lot of labs use the Pathvysion kit off label and dilute the probe and change other things from the original FDA approved protocol. Then again, even deparaffinzing the slide using xylene is off label, since it was approved using Hemo-D substitute. I imagine it would be harder to go off label with Ventana's software. I guess you've got to weigh the pros and cons as with everything. Mark Tarango On Sun, Jul 3, 2011 at 2:28 AM, Gudrun Lang gu.l...@gmx.at wrote: The Her2 CB11 clone of Ventana is FDA approved. In the package insert of the Her2 4B5 clone is stated, that the antibody was compared to CB11 and shows same or better performance. The CB11 clone was exchanged through 4B5. On the NordiQC website Ventana 4B5 and Dako Herceptest are called FDA approved. http://www.nordiqc.org/Run-30-B10/Assessment/assessment-B10-HER2.htm So in my opinion the company wouldn't exchange its antibody, if it wouldn't be approved now or soon. Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Patsy Ruegg Gesendet: Samstag, 02. Juli 2011 18:09 An: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] VENTANA ULTRA ER,PR,HER2 I thought Ventana and Dako both had FDA approved Her2, I know Dako has Hercept Test. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Tuesday, June 28, 2011 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VENTANA ULTRA ER,PR,HER2 We are investigating getting the Ventana Ultra. I discovered that the ER, PR, HER2 are not yet FDA approved. If you are using the Ventana Ultra how are you doing the ER, PR, HER2? Do you use the Benchmark XT? Is anyone using the INFORM HER2 Dual ISH DNA Probe Cocktail Assay? ANTOINETTE CRILL, E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] VENTANA ULTRA ER,PR,HER2
I thought Ventana and Dako both had FDA approved Her2, I know Dako has Hercept Test. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Tuesday, June 28, 2011 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VENTANA ULTRA ER,PR,HER2 We are investigating getting the Ventana Ultra. I discovered that the ER, PR, HER2 are not yet FDA approved. If you are using the Ventana Ultra how are you doing the ER, PR, HER2? Do you use the Benchmark XT? Is anyone using the INFORM HER2 Dual ISH DNA Probe Cocktail Assay? ANTOINETTE CRILL, E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] VENTANA ULTRA ER,PR,HER2
Hi Antoinette, We currently use Ventana's ER and PR on both XT and Ultra. From what I understand, the antibody dispenser is labeled IVD. We report all cases as being 'approved by the FDA for IVD use'. We do perform correlation/precision studies twice a year. For this study we run five cases on both the XT and an ultra and document that the result comes out the same. I have actually found that this is a compliance issue with Joint Commission. I can try to find the reference if you need it. It states that if you are running the same test on two different platforms, you must prove correlation. As far as the Her2 dual ISH, we are in the process of evaluating it now. I see it as a definite advantage that it is bright field, permanent, and has the potential to dramatically increase TAT. We are in the middle of a cost analysis. On the other hand, we currently run Pathvysion and I see that some literature calls FISH and pathvysion the 'gold standard'. This is the most highly scrutinized test we perform considering the CAP/ASCO guidelines. My gut also tells me...why take a chance with this when our current system is not 'broken'? I did find that CAP offers two mailings for profiency testing using bright field ISH. Let me know how you make out. Hope this helps. Melissa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Tuesday, June 28, 2011 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VENTANA ULTRA ER,PR,HER2 We are investigating getting the Ventana Ultra. I discovered that the ER, PR, HER2 are not yet FDA approved. If you are using the Ventana Ultra how are you doing the ER, PR, HER2? Do you use the Benchmark XT? Is anyone using the INFORM HER2 Dual ISH DNA Probe Cocktail Assay? ANTOINETTE CRILL, E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VENTANA ULTRA ER,PR,HER2
We are investigating getting the Ventana Ultra. I discovered that the ER, PR, HER2 are not yet FDA approved. If you are using the Ventana Ultra how are you doing the ER, PR, HER2? Do you use the Benchmark XT? Is anyone using the INFORM HER2 Dual ISH DNA Probe Cocktail Assay? ANTOINETTE CRILL, E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] VENTANA ULTRA ER,PR,HER2
We will be going through this transition, here at Yuma Regional Medical Center.. Call me if you have questions Jesus Ellin 928-336-1743 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Tuesday, June 28, 2011 7:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VENTANA ULTRA ER,PR,HER2 We are investigating getting the Ventana Ultra. I discovered that the ER, PR, HER2 are not yet FDA approved. If you are using the Ventana Ultra how are you doing the ER, PR, HER2? Do you use the Benchmark XT? Is anyone using the INFORM HER2 Dual ISH DNA Probe Cocktail Assay? ANTOINETTE CRILL, E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana ultra
Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana ultra
Please Reply All as I'd like to hear feedback also. Thanks. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Wednesday, April 13, 2011 8:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana ultra
I've heard that adding slides and/or reagents can add as much as 8 minutes to the run time. Again, that's just what I've heard, don't know if it's fact or fiction ~Kim~ OU ROCKS ~Don't be afraid your life will end, be afraid it will never begin~ --- On Wed, 4/13/11, Sebree Linda A lseb...@uwhealth.org wrote: From: Sebree Linda A lseb...@uwhealth.org Subject: RE: [Histonet] Ventana ultra To: barbara.cr...@lpnt.net, histonet@lists.utsouthwestern.edu Date: Wednesday, April 13, 2011, 2:09 PM Please Reply All as I'd like to hear feedback also. Thanks. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Wednesday, April 13, 2011 8:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana ultra
each of the 30 drawers essentially is it's own run. You don't have to wait for all 30 drawers to finish their runs before adding more slides. There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! barbara.cr...@lpnt.net 4/13/2011 9:39 AM Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana ultra
I currently have one XT and 3 Ultras. I love the Ultras. There is no difference in reagent costs. The only thing that I can say remotely related is that if you keep both platforms, the XT and the ultras have separate CC1, CC2, and LCS. I am fairly certain the contents of the bottle are the same, but the label and part numbers are different. I love the continuous access. I have it set up where the three ultras are 'top loaded' with set antibodies every day. This eliminates bouncing things around all the time. Contact me if you would like anymore info. I would be happy to help. I notice no delay in run times. melissa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Wednesday, April 13, 2011 9:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana ultra
I recently got an Ultra about a month ago and doing test runs, I have really enjoyed the continuous feed as well but I have a question...if I deactivate a product from the Ultra's inventory list I notice it's still in my XT's inventory list. So, I have to deactivate everything off of each instrument? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa Sent: Wednesday, April 13, 2011 9:45 AM To: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventana ultra I currently have one XT and 3 Ultras. I love the Ultras. There is no difference in reagent costs. The only thing that I can say remotely related is that if you keep both platforms, the XT and the ultras have separate CC1, CC2, and LCS. I am fairly certain the contents of the bottle are the same, but the label and part numbers are different. I love the continuous access. I have it set up where the three ultras are 'top loaded' with set antibodies every day. This eliminates bouncing things around all the time. Contact me if you would like anymore info. I would be happy to help. I notice no delay in run times. melissa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of barbara.cr...@lpnt.net Sent: Wednesday, April 13, 2011 9:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana ultra
We like ours as well. It's not as continual as we originally thought to maximize its potential I would recommend placing frequently used antibodies on the wheel with the first run. As long as you have the antibody you want to stain for on the wheel you can add the slide anytime you want and it will run it. If the antibody is not on the wheel you have to set a landing zone in order to add it. This usually works out fine, however once the run that is already going reaches a certain point, you aren't able to set a landing zone and may have to wait awhile to load new slides. It is pretty cool that you can run FITC, FISH, and IHC at the same time (however we tend to put our EBER on overnight because it's a fairly long run). Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, April 13, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu; barbara.cr...@lpnt.net Subject: Re: [Histonet] Ventana ultra each of the 30 drawers essentially is it's own run. You don't have to wait for all 30 drawers to finish their runs before adding more slides. There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! barbara.cr...@lpnt.net 4/13/2011 9:39 AM Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Ventana ultra
What we like about the Ultra is, that there's no need for cleaning runs between the runs and that you can take out the bulk and waste containers during the run. We don't use the continious loading, because it doesn't fit to our workplan and because we use various antibodies from run to run. The runs are faster, but more than 2 runs per 8 hour shift and 1 run over night are not possible for us. That's the same workload we did with the XT. There are windows during the run, when you can add new slides and reagens. If the needed reagens in on the instrument, you can add them any time, if there's enough place. But the run will last longer and you have to wait for a window of the longer run to get the reagens off the instrument. Ready stained slides can be taken out independently. But the free pads cannot be filled and runs started, until you have the possibility to add the right reagens. So we decided to stay with patch-workload and real go-away. Bye Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von barbara.cr...@lpnt.net Gesendet: Mittwoch, 13. April 2011 15:39 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Ventana ultra Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana ultra
We load our Ultras with the most commonly used antibodies each morning. So that minimizes the number of landing zones we need to use. Also, (if you run both XTs and Ultras) run your antibodies with the longest protocols on Ultra and that will shorten your XT runs. Setlak, Lisa lset...@childrensmemorial.org 4/13/2011 10:54 AM We like ours as well. It's not as continual as we originally thought to maximize its potential I would recommend placing frequently used antibodies on the wheel with the first run. As long as you have the antibody you want to stain for on the wheel you can add the slide anytime you want and it will run it. If the antibody is not on the wheel you have to set a landing zone in order to add it. This usually works out fine, however once the run that is already going reaches a certain point, you aren't able to set a landing zone and may have to wait awhile to load new slides. It is pretty cool that you can run FITC, FISH, and IHC at the same time (however we tend to put our EBER on overnight because it's a fairly long run). Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, April 13, 2011 9:42 AM To: histonet@lists.utsouthwestern.edu; barbara.cr...@lpnt.net Subject: Re: [Histonet] Ventana ultra each of the 30 drawers essentially is it's own run. You don't have to wait for all 30 drawers to finish their runs before adding more slides. There are 2 reagents that are exclusive to the Ultra and they are a little more expensive. Ultra CC1 and Ultra LCS,(and ultra CC2, if you use CC2). The EZ Prep, Reaction buffer and SSC are the same reagents that you use on your XT. The software can be a little quirky sometimes. But, overall, we really like ours because of the continuous feed ability. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! barbara.cr...@lpnt.net 4/13/2011 9:39 AM Does anyone use the Benchmark Ultra - care to share your comments? We are considering switching from the Benchmark XT to the Ultra but would like to hear from users about this instrument. Is there an increase in reagent cost? Can you really add more slides without adding time to the run? ANTOINETTE CRILL, MBA,CT(ASCP) TEAM LEADER ANATOMIC PATHOLOGY DANVILLE REGIONAL MEDICAL CENTER (O) 434.799.4470 (F) 434.773.6806 E-mail: barbara.cr...@lpnt.netmailto:barbara.cr...@lpnt.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. - This message was secured by ZixCorp(R). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Ultra
We are in the process of upgrading our immuno stainers to the Ventana Ultra. I am interested in how people validate a new instrument and how the Ultra was integrated or changed lab workflow. Cathy Kelowna, B.C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Ultra users
Hi all, Could those of you who use Ultras in your labs, please contact me offline? I am taking an unofficial survey. Thank you, Noelle Noёlle Linke M.S., HTL(ASCP)QIHC Manager, Histology Services UCLA Department of Pathology and Laboratory Medicine Phone: 310-825-7397 Fax: 310-983-3289 nli...@mednet.ucla.edumailto:nli...@mednet.ucla.edu Pager: 97471 IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana ULTRA Immuno Stainer
Excellent stainer, but you need to look at your process to help facilitate the technology. if you have nay questions you can reach me. Jesus Ellin Yuma Regional Medical Center 928-336-1144 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu on behalf of Gomez, Milton Sent: Thu 9/10/2009 7:35 PM To: Histonet Subject: [Histonet] Ventana ULTRA Immuno Stainer Dear Histonetters, Is anyone out there using the Ventana ULTRA Immuno Stainer? What is your experience with them? What are the advantages, disadvantages, pros and cons? Thank you very much in advance? Milton A. Gomez, HTL (ASCP) Technical Supervisor Immunohistochemistry Department ARUP Laboratories, Inc. 500 Chipeta Way Salt Lake City, UT 84108-1221 Desk Phone: 801-583-2787, ext.3869 Lab. Phone: 801-584-5257/5242 Fax: 801-584-5217 E-mail: milton.go...@aruplab.com https://connect.yumaregional.org/CitrixFEI/composemessage.asp?to=milton.go...@aruplab.com Web: www.aruplab.com http://www.aruplab.com/ - -- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana ULTRA Immuno Stainer
Dear Histonetters, Is anyone out there using the Ventana ULTRA Immuno Stainer? What is your experience with them? What are the advantages, disadvantages, pros and cons? Thank you very much in advance? Milton A. Gomez, HTL (ASCP) Technical Supervisor Immunohistochemistry Department ARUP Laboratories, Inc. 500 Chipeta Way Salt Lake City, UT 84108-1221 Desk Phone: 801-583-2787, ext.3869 Lab. Phone: 801-584-5257/5242 Fax: 801-584-5217 E-mail: milton.go...@aruplab.com mailto:milton.go...@aruplab.com Web: www.aruplab.com http://www.aruplab.com/ - -- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
