Hello everyone, A few people asked me to post some information on my West Nile Virus IHC test method, so here are some general comments. If you'd like more detailed information, please write to me directly.
Tissue pretreatment - pressure cooker (Biocare Decloaking Chamber) with Target Retrieval solution (Dako) Primary antibody - anti-West Nile Virus (BioReliance, cat. # 81-002; clone 7H2; working dilution at 3-6 ug/ml Ab concentration, depending upon lot) Secondary linking antibody - EnVision+/HRP goat anti-mouse IgG (Dako; with 2-4% normal case species serum added per volume) Chromogen - AEC+ (Dako) Best regards, Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
