Re: [Histonet] Water Quality from Lab Gen Checklist distilled vs deionized vs reagent grade water

[Morken, Timothy via Histonet]

Renee, for practical purposes in histology they are pretty much the same. Note 
that the original water quality is key because certain things can carry over in 
each type of system. Commercial suppliers do all the filtering, conditioning 
and finishing for you. 

Deionized (DI) is now the usual water produced in institutions because it is 
easier to maintain and produces adequate amounts of water. Distillation 
requires a still and produces relatively small quantities compared to DI.  In 
our histo lab we have a DI system, but it would be over-kill for a small lab. 
Buying distilled water will be fine. The company that makes distilled water 
does all the filtering and finishing for you so it is pure. If really concerned 
you can buy from a scientific supplier rather than a grocery store. In our EM 
lab we make up muscle histochemistry stains and  with type 1 water we buy in 1 
liter bottles. We have a DI system for everything else. 

This is a good article on the difference between DI and Dist. Water:

http://www.labmanager.com/laboratory-technology/2014/09/the-right-water#.WdZwx4TyuUk


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Renee H. Workman via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, October 05, 2017 10:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Water Quality from Lab Gen Checklist distilled vs deionized 
vs reagent grade water

Going over checklist needed input on water quality.  We are a small lab and use 
distilled water purchased commercially.  We do IHC but no special stains.  We 
use the water in our water baths and for preparing IHC buffer.  Should we 
upgrade to deionized or use distilled?

Renee H. Workman
Histology Supervisor
Virginia Urology
9105 Stony Point Drive
Richmond, VA  23235
W: 804-527-1316 | F: 804-270-0917
rhwork...@uro.com<mailto:rhwork...@uro.com> | www.uro.com<http://www.uro.com>





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[Histonet] Water Quality from Lab Gen Checklist distilled vs deionized vs reagent grade water

[Renee H. Workman via Histonet]

Going over checklist needed input on water quality.  We are a small lab and use 
distilled water purchased commercially.  We do IHC but no special stains.  We 
use the water in our water baths and for preparing IHC buffer.  Should we 
upgrade to deionized or use distilled?

Renee H. Workman
Histology Supervisor
Virginia Urology
9105 Stony Point Drive
Richmond, VA  23235
W: 804-527-1316 | F: 804-270-0917
rhwork...@uro.com | www.uro.com





Disclaimer: The email and files transmitted with it are confidential and are 
intended solely for the use of the individual or entity to whom they are 
addressed. If you are not the original recipient or the person responsible for 
the delivering the email to the intended recipient, be advised that you have 
received this email in error, and that any use, dissemination, forwarding, 
printing or copying of this email is strictly prohibited. If you received this 
email in error, please delete it from your system without copying it, and 
notify the sender by reply email so that our address record can be corrected.

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