Re: [Histonet] West Nile IHC
Tresa, I've been staining for WNV for many years and we've had a number of papers published with our results. I'm currently at the NSH symnposium, but will forward our SOP to you when I return on Thursday. Just a quick note, though. Although we've had many positive results with birds (esp. raptors), I've only seen one positive cell in all the horses that I've tried staining. So the fact that you haven't had any positive equine tissues does not surprise me. It could be that the horse is an end-host of the virus, so there is no replication going on. The virus might be being degraded by cellular processes and thus, not recognized by the antibody. Jan Shivers Section Head IHC/Histo/EM Veterinary Diagnostic Laboratory University of Minnesota St. Paul, MN On Sep 24 2010, Goins, Tresa wrote: After many frustrating attempts, I would greatly appreciate any information on the successful application of IHC for the detection of West Nile Virus in fixed tissue. I have tried no retrieval, enzymatic and heat retrieval with no success on horse and bird tissues. Would someone please share successful protocols (retrieval) and antibody sources for equine and avian fixed tissues? Our tissues are formalin fixed and the detection method used is biotin-streptavidin, alkaline phosphatase with a new fuchsin chromogen (Vulcan Fast Red) Thank you in advance, Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] West Nile IHC
Hello Jan Could you please post this protocol on histonet, West Nile IHC testing is something out lab is definitely interested in pursuing. Much appreciated, and enjoy the S/C in Seattle! Kathy 09/27/10 2:51 AM Tresa, I've been staining for WNV for many years and we've had a number of papers published with our results. I'm currently at the NSH symnposium, but will forward our SOP to you when I return on Thursday. Just a quick note, though. Although we've had many positive results with birds (esp. raptors), I've only seen one positive cell in all the horses that I've tried staining. So the fact that you haven't had any positive equine tissues does not surprise me. It could be that the horse is an end-host of the virus, so there is no replication going on. The virus might be being degraded by cellular processes and thus, not recognized by the antibody. Jan Shivers Section Head IHC/Histo/EM Veterinary Diagnostic Laboratory University of Minnesota St. Paul, MN On Sep 24 2010, Goins, Tresa wrote: After many frustrating attempts, I would greatly appreciate any information on the successful application of IHC for the detection of West Nile Virus in fixed tissue. I have tried no retrieval, enzymatic and heat retrieval with no success on horse and bird tissues. Would someone please share successful protocols (retrieval) and antibody sources for equine and avian fixed tissues? Our tissues are formalin fixed and the detection method used is biotin-streptavidin, alkaline phosphatase with a new fuchsin chromogen (Vulcan Fast Red) Thank you in advance, Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] West Nile IHC
After many frustrating attempts, I would greatly appreciate any information on the successful application of IHC for the detection of West Nile Virus in fixed tissue. I have tried no retrieval, enzymatic and heat retrieval with no success on horse and bird tissues. Would someone please share successful protocols (retrieval) and antibody sources for equine and avian fixed tissues? Our tissues are formalin fixed and the detection method used is biotin-streptavidin, alkaline phosphatase with a new fuchsin chromogen (Vulcan Fast Red) Thank you in advance, Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] West Nile
Has anyone found a good antibody for west Nile FFPE that will work on horses? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
