RE: [Histonet] Which fixative for eyes
Davidson's fixative. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron Sent: Wednesday, January 16, 2013 11:49 AM To: Lucie Guernsey; Louro, Pedro Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Which fixative for eyes We work with mice and do a variety of fixatives, but we prefer a methanol/acetic acid fixative. It holds the shape of the eye very well, although it may not show the Schlemm's canal as well as other fixatives. -Liz -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Wednesday, January 16, 2013 1:59 PM To: Louro, Pedro Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Which fixative for eyes I'm curious about this as well. We work with mice and rats in our lab and whenever we need eyes (corneas, more specifically), we immersion fix them whole in Bouin's solution overnight at 4C then transfer to 70% EtOH until we're ready to excise the corneas, process and embed into paraffin. We've found that we get nice half-moon sections with good looking stromal and epithelial layers this way compared to fixing the eyes in 4% PFA (which yielded squiggly, damaged corneas). We then perform IHC. Would love to know if there's a better way, even though this seems to work for us. Thanks, Lucie Lucie Guernsey UCSD lguern...@ucsd.edu On Wed, Jan 16, 2013 at 10:32 AM, Louro, Pedro lou...@princeton.huntingdon.com wrote: Hello to the eye experts, I am currently working on a large animal (LA) eye project which will give me the flexibility of experimenting with different fixatives. I am currently using Modified Davidson's solution (48 hours) followed by a 10% NBF solution prior to trimming the eyes, embedding , microtomy and HE staining. After literature research, I found that different labs are using: Modified Davidson's solution Davidson's solution 10% NBF Gluteraldehyde Some places inject the eye with fixative before submerging the eye itself. I wanted to get some ideas / preferences from the experts out there so I can cover my entire basis before finalizing my conclusions. Thanks in advance to all, Pedro Louro Group Leader Histology / IHC ** ** Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect ** ** LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Which fixative for eyes
Dear Pedro Louro We are using Alcoholic formalin fixative for ocular (Eye) specimens, Reference Tech Sample (ASAP) HT-2(1995), since last 19 year without facing any problem. Composition: Ethanol absolute 90 ml Formaldehyde con 10 ml Fix for 24 hours Thanks, Muhammad Tahseen Histology Supervisor SKMCHRC Lahore Pakistan Hello to the eye experts, I am currently working on a large animal (LA) eye project which will give me the flexibility of experimenting with different fixatives. I am currently using Modified Davidson's solution (48 hours) followed by a 10% NBF solution prior to trimming the eyes, embedding , microtomy and HE staining. After literature research, I found that different labs are using: Modified Davidson's solution Davidson's solution 10% NBF Gluteraldehyde Some places inject the eye with fixative before submerging the eye itself. I wanted to get some ideas / preferences from the experts out there so I can cover my entire basis before finalizing my conclusions. Thanks in advance to all, Pedro Louro Group Leader Histology / IHC Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Which fixative for eyes
Hello to the eye experts, I am currently working on a large animal (LA) eye project which will give me the flexibility of experimenting with different fixatives. I am currently using Modified Davidson's solution (48 hours) followed by a 10% NBF solution prior to trimming the eyes, embedding , microtomy and HE staining. After literature research, I found that different labs are using: Modified Davidson's solution Davidson's solution 10% NBF Gluteraldehyde Some places inject the eye with fixative before submerging the eye itself. I wanted to get some ideas / preferences from the experts out there so I can cover my entire basis before finalizing my conclusions. Thanks in advance to all, Pedro Louro Group Leader Histology / IHC Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Which fixative for eyes
I'm curious about this as well. We work with mice and rats in our lab and whenever we need eyes (corneas, more specifically), we immersion fix them whole in Bouin's solution overnight at 4C then transfer to 70% EtOH until we're ready to excise the corneas, process and embed into paraffin. We've found that we get nice half-moon sections with good looking stromal and epithelial layers this way compared to fixing the eyes in 4% PFA (which yielded squiggly, damaged corneas). We then perform IHC. Would love to know if there's a better way, even though this seems to work for us. Thanks, Lucie Lucie Guernsey UCSD lguern...@ucsd.edu On Wed, Jan 16, 2013 at 10:32 AM, Louro, Pedro lou...@princeton.huntingdon.com wrote: Hello to the eye experts, I am currently working on a large animal (LA) eye project which will give me the flexibility of experimenting with different fixatives. I am currently using Modified Davidson's solution (48 hours) followed by a 10% NBF solution prior to trimming the eyes, embedding , microtomy and HE staining. After literature research, I found that different labs are using: Modified Davidson's solution Davidson's solution 10% NBF Gluteraldehyde Some places inject the eye with fixative before submerging the eye itself. I wanted to get some ideas / preferences from the experts out there so I can cover my entire basis before finalizing my conclusions. Thanks in advance to all, Pedro Louro Group Leader Histology / IHC Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Which fixative for eyes
We work with mice and do a variety of fixatives, but we prefer a methanol/acetic acid fixative. It holds the shape of the eye very well, although it may not show the Schlemm's canal as well as other fixatives. -Liz -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Wednesday, January 16, 2013 1:59 PM To: Louro, Pedro Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Which fixative for eyes I'm curious about this as well. We work with mice and rats in our lab and whenever we need eyes (corneas, more specifically), we immersion fix them whole in Bouin's solution overnight at 4C then transfer to 70% EtOH until we're ready to excise the corneas, process and embed into paraffin. We've found that we get nice half-moon sections with good looking stromal and epithelial layers this way compared to fixing the eyes in 4% PFA (which yielded squiggly, damaged corneas). We then perform IHC. Would love to know if there's a better way, even though this seems to work for us. Thanks, Lucie Lucie Guernsey UCSD lguern...@ucsd.edu On Wed, Jan 16, 2013 at 10:32 AM, Louro, Pedro lou...@princeton.huntingdon.com wrote: Hello to the eye experts, I am currently working on a large animal (LA) eye project which will give me the flexibility of experimenting with different fixatives. I am currently using Modified Davidson's solution (48 hours) followed by a 10% NBF solution prior to trimming the eyes, embedding , microtomy and HE staining. After literature research, I found that different labs are using: Modified Davidson's solution Davidson's solution 10% NBF Gluteraldehyde Some places inject the eye with fixative before submerging the eye itself. I wanted to get some ideas / preferences from the experts out there so I can cover my entire basis before finalizing my conclusions. Thanks in advance to all, Pedro Louro Group Leader Histology / IHC ** ** Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect ** ** LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
