Re: [Histonet] Whole mount histology
Don't know how fast you need this information, but there will be a NSH teleconference on this topic on May 25, 2011. www.nsh.org 443-535-4060 Peggy A. Wenk. HTL(ASCP)SLS NSH Teleconference coordinator (Disclaimer: volunteer position, so don't get paid if anyone signs up) -- From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Sent: Monday, January 17, 2011 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Whole mount histology I am totally unfamiliar regarding whole mount histology as it pertains to prostate histology. Can anyone in histoland assist me in finding information out regarding more specifics, especially in lieu of what costs would be incurred to implement this in a routine hospital histology department. Appreciate any and all guidance!! Dorothy Webb, HT Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Whole mount histology
I am totally unfamiliar regarding whole mount histology as it pertains to prostate histology. Can anyone in histoland assist me in finding information out regarding more specifics, especially in lieu of what costs would be incurred to implement this in a routine hospital histology department. Appreciate any and all guidance!! Dorothy Webb, HT Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Whole mount histology
Dorothy: First of all you have to be sure that the micrtotome you are going to use has enough cutting path to cover the embedded specimen. You will also need the so called mega cassettes and mega molds, unless you want to make them yourself out of plastic containers (for the cassettes) and of heavy aluminum foil (for the molds) as I first did in 1990 when I started the procedure at my laboratory. Instead of the molds you could use Leukhard's rectangles. Finally you have to develop a fixation and processing protocol suitable for your specimens. René J. --- On Mon, 1/17/11, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Subject: [Histonet] Whole mount histology To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Date: Monday, January 17, 2011, 10:51 AM I am totally unfamiliar regarding whole mount histology as it pertains to prostate histology. Can anyone in histoland assist me in finding information out regarding more specifics, especially in lieu of what costs would be incurred to implement this in a routine hospital histology department. Appreciate any and all guidance!! Dorothy Webb, HT Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Whole mount histology
Hi Dorothy, I concur with what Rene has mentioned. Main cost would be microtome-related, if you don't have microtomes that can handle mega cassettes. Other process-related costs i.e. fixation, tech time, supplies wouldn't be much different once process was optimized. In fact, tech time is likely reduced, as it may be quicker (depending on the tech) to cut 4-6 mega blocks than 18-24 regular blocks. You'll also have to stain and coverslip the slides creatively, as they may not fit on your stainer. Our pathologists did many cases, but eventually went with regular cassettes and processing. It's a nice procedure to have available. Email me if you need more information i.e. specifics on supplies/process. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
