Re: [Histonet] b-cells stain
Thanks John, Since I was writing the message about Gomori's chrome alum hematoxylin without the procedure in front of me yesterday (not the best idea, I know) I didn't have the whole formulation available to me. For Gomori's chrome alum hematoxylin, in addition to potassium dichromate, there is also chromium potassium sulfate and sulfuric acid as well. By no means is there any ferric chloride in it and as I mentioned if one tried to make this with ferric chloride, I doubt it would work for beta cells since according to Bancroft in *Theory Practice of Histotechnology* chrome alum has a strong affinity for sulfonic acid radicals which are produced in the oxidation of the glycoproteins in the beta cells in earlier steps of the stain. So long story short: ferric chloride probably would not work for Gomori's stain. Sorry if it sounded like I suggested it would. I really appreciate having folks like John Kiernan around to point out possible mis-communications like that. Have a great weekend everyone, Amos On Fri, Dec 2, 2011 at 2:04 AM, John Kiernan jkier...@uwo.ca wrote: Dear Amos, You may have confused some histonetters if any of them try to make up Gomori's chrome alum hematoxylin with either potassium dichromate or ferric chloride! Cheers, John Kiernan Anatomy, UWO London, Canada = = = On 01/12/11, *Amos Brooks *amosbro...@gmail.com wrote: Hi, I like to prepare solutions myself as well. As you say though paraldehyde is classified as a drug so there are hoops to jump through to be able to purchase it. This is one of those times that it totally makes sense to let someone else make it up. EMS sells aldehyde fuchsin so it saves me the hassle of dealing with the DEA. Better yet the price isn't terrible and works great. Here's the info for it: RT 26096 Aldehyde Fuchsin Solution 250 ml 24.00 http://www.emsdiasum.com/microscopy/products/histology/staining_solutions.aspx#26091 Now as for Gomori's chrome alum hematoxylin, you really are better off using the chrome (potassium dichromate if I recall correctly) than ferric chloride (Weigerts formulation right?). I don't think the iron hematoxylin would be as specific to the beta cells, but hey, if you decide to try it, please let me know if it works out for you. Ferric chloride is better to work with than potassium dichromate! Heck you could write up a new procedure and name it after yourself. Amos On Thu, Dec 1, 2011 at 1:00 PM, histonet-requ...@lists.utsouthwestern.eduwrote: Message: 3 Date: Thu, 1 Dec 2011 06:25:00 -0800 (PST) From: Silvina Molinuevo silvinamolinu...@yahoo.com.ar Subject: [Histonet] b-cells stain To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 1322749500.19574.yahoomail...@web113609.mail.gq1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Hi Histonetters!! I want to do Chrome Hematoxylin-Phloxin (from Gomori) to show b-cells. Does anybody know if the stain go well with ferrum hematoxylin instead of chrome hematoxylin? I know that the best stain is Aldehyde-fuchsin but I can't import paraldehyde because of legal restrictions about that product in my country. Thanks in advance. Dr María Silvina Molinuevo Grupo de Investigacion en Osteopatias y Metabolismo Mineral Departamento de Ciencias Biologicas Facultad de Ciencias Exactas Universidad Nacional de La Plata 47 y 115 (1900)La Plata Argentina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] b-cells stain
Hi Histonetters!! I want to do Chrome Hematoxylin-Phloxin (from Gomori) to show b-cells. Does anybody know if the stain go well with ferrum hematoxylin instead of chrome hematoxylin? I know that the best stain is Aldehyde-fuchsin but I can't import paraldehyde because of legal restrictions about that product in my country. Thanks in advance. Dr María Silvina Molinuevo Grupo de Investigacion en Osteopatias y Metabolismo Mineral Departamento de Ciencias Biologicas Facultad de Ciencias Exactas Universidad Nacional de La Plata 47 y 115 (1900)La Plata Argentina www.biol.unlp.edu.ar/giomm e-mail: silvina.molinu...@bigfoot.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] b-cells stain
Greetings Silvina: You do not have to use paraldehyde, you can make a perfectly good aldehyde fuchsin using acetaldehyde since one molecule of paraldehyde depolymerizes into 3 molecules of acetaldehyde. Just use 3x the amount of acetaldehyde. n-butyraldehyde will also work for b-cells. See Buehner et al. J.Histochem. Cytochem. 27(3):782787, 1979 for more thorough discussion of the chemistry. And, if a ferrous hematoxylin worked for b-cells we would not need the chrome alum variety. Geoff On 12/1/2011 9:25 AM, Silvina Molinuevo wrote: Hi Histonetters!! I want to do Chrome Hematoxylin-Phloxin (from Gomori) to show b-cells. Does anybody know if the stain go well with ferrum hematoxylin instead of chrome hematoxylin? I know that the best stain is Aldehyde-fuchsin but I can't import paraldehyde because of legal restrictions about that product in my country. Thanks in advance. Dr María Silvina Molinuevo Grupo de Investigacion en Osteopatias y Metabolismo Mineral Departamento de Ciencias Biologicas Facultad de Ciencias Exactas Universidad Nacional de La Plata 47 y 115 (1900)La Plata Argentina www.biol.unlp.edu.ar/giomm e-mail: silvina.molinu...@bigfoot.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] b-cells stain
Hi, I like to prepare solutions myself as well. As you say though paraldehyde is classified as a drug so there are hoops to jump through to be able to purchase it. This is one of those times that it totally makes sense to let someone else make it up. EMS sells aldehyde fuchsin so it saves me the hassle of dealing with the DEA. Better yet the price isn't terrible and works great. Here's the info for it: RT 26096 Aldehyde Fuchsin Solution 250 ml 24.00 http://www.emsdiasum.com/microscopy/products/histology/staining_solutions.aspx#26091 Now as for Gomori's chrome alum hematoxylin, you really are better off using the chrome (potassium dichromate if I recall correctly) than ferric chloride (Weigerts formulation right?). I don't think the iron hematoxylin would be as specific to the beta cells, but hey, if you decide to try it, please let me know if it works out for you. Ferric chloride is better to work with than potassium dichromate! Heck you could write up a new procedure and name it after yourself. Amos On Thu, Dec 1, 2011 at 1:00 PM, histonet-requ...@lists.utsouthwestern.eduwrote: Message: 3 Date: Thu, 1 Dec 2011 06:25:00 -0800 (PST) From: Silvina Molinuevo silvinamolinu...@yahoo.com.ar Subject: [Histonet] b-cells stain To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 1322749500.19574.yahoomail...@web113609.mail.gq1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Hi Histonetters!! I want to do Chrome Hematoxylin-Phloxin (from Gomori) to show b-cells. Does anybody know if the stain go well with ferrum hematoxylin instead of chrome hematoxylin? I know that the best stain is Aldehyde-fuchsin but I can't import paraldehyde because of legal restrictions about that product in my country. Thanks in advance. Dr María Silvina Molinuevo Grupo de Investigacion en Osteopatias y Metabolismo Mineral Departamento de Ciencias Biologicas Facultad de Ciencias Exactas Universidad Nacional de La Plata 47 y 115 (1900)La Plata Argentina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] b-cells stain
Acetaldehyde can be used instead of paraldehyde, to make aldehyde fuchsin. Substitute 1.5 - 2.0 mL acetaldehyde for every 1.0 mL of paraldehyde. Acetaldehyde is usually not a restricted drug, a whole lot cheaper than acetaldehyde, and once opened, remains good for much longer than paraldehyde, when stored in the refrigerator (at least 18 months instead for the 2-3 months for paraldehyde). The aldehyde-fuchsin, once made, will only be good for about 3-4 weeks, when stored in the refrig, regardless of whether it was made with paraldehyde or acetaldehyde. The source for the exchange was this article: Gabe, M. 1953. Sur quelques applications de la coloration par la fuchsine-paraldehyde. Bull. Micros. Appl. Ser. 2, 3: 153-162. One of my students, Yelena Mushkina, HTL(ASCP), translated it from the French to her native Russian, and then from Russian to English. Took a couple of dictionaries! I wrote this up in a Letter to the Editor in the Dec. 1996 NSH Journal of Histotechnology Acetaldehyde as a Substitute for Paraldehyde. In case you need some references. IHC is actually better for demonstrating beta cells - whether for pituitary or for pancreas. Just something else to think about. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -Original Message- From: Silvina Molinuevo Sent: Thursday, December 01, 2011 9:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] b-cells stain Hi Histonetters!! I want to do Chrome Hematoxylin-Phloxin (from Gomori) to show b-cells. Does anybody know if the stain go well with ferrum hematoxylin instead of chrome hematoxylin? I know that the best stain is Aldehyde-fuchsin but I can't import paraldehyde because of legal restrictions about that product in my country. Thanks in advance. Dr María Silvina Molinuevo Grupo de Investigacion en Osteopatias y Metabolismo Mineral Departamento de Ciencias Biologicas Facultad de Ciencias Exactas Universidad Nacional de La Plata 47 y 115 (1900)La Plata Argentina www.biol.unlp.edu.ar/giomm e-mail: silvina.molinu...@bigfoot.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
