Re: [Histonet] Centrifuge for cytospins
Thanks for sharing Joe! :) Jessica -Original Message- From: Joe W. Walker, Jr. [mailto:joewal...@rrmc.org] Sent: Tuesday, April 28, 2015 2:31 PM To: Cooper, Brian; Piche, Jessica; histonet@lists.utsouthwestern.edu Subject: RE: Centrifuge for cytospins In all of my experiences with cytology preparations, the first step would be to centrifuge the specimen. This concentrates the cells for the next step; cytocentrifuge, which is used for the presentation of the cells for evaluation. Here is an excerpt from our procedure: 3. Label a 50 ml centrifuge tube with the cytology accession label. 4. Mix the specimen using a vortex mixer for 10-30 seconds. 5. Pour contents of specimen from the original container into the 50 ml centrifuge tube and cap the tube. 6. Centrifuge the specimen at 600g for 10 minutes (program 1) or 1200g for 5 minutes (program 2). 7. Pour off the supernatant and re-suspend the sediment in 0.5 to 1ml of Cytospin (green) fluid, depending on the cell button size. Evaluating the number of drops you might need for your cytocentrifuge can be performed by taking a crop from the sediment, placing it on a slide, placing a 24x50mm cover glass on the specimen and then observing the cellularity of the specimen. I have document that illustrates this but I don't think the list serve will allow attachments. You can email me privately for this document. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewal...@rrmc.orgwww.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Tuesday, April 28, 2015 12:10 PM To: Piche, Jessica; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Centrifuge for cytospins We make our BAL cytospins directly--no centrifuging first. Brian -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Tuesday, April 28, 2015 8:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Centrifuge for cytospins Hi Everyone, Just asking a quick question for our Cyto department. Are BAL slides centrifuged and then made in to cytospin slides or do you just make the cytospin slides with no centrifuging? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged
Re: [Histonet] Centrifuge for cytospins
Thanks for sharing Brian. Jessica -Original Message- From: Cooper, Brian [mailto:bcoo...@chla.usc.edu] Sent: Tuesday, April 28, 2015 12:10 PM To: Piche, Jessica; histonet@lists.utsouthwestern.edu Subject: RE: Centrifuge for cytospins We make our BAL cytospins directly--no centrifuging first. Brian -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Tuesday, April 28, 2015 8:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Centrifuge for cytospins Hi Everyone, Just asking a quick question for our Cyto department. Are BAL slides centrifuged and then made in to cytospin slides or do you just make the cytospin slides with no centrifuging? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Centrifuge for cytospins
Hi Everyone, Just asking a quick question for our Cyto department. Are BAL slides centrifuged and then made in to cytospin slides or do you just make the cytospin slides with no centrifuging? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] centrifuge?
In case anyone was wondering, you do need to rebalance it. However, that actually means level it, which is what I was going to do anyway! I was thinking some inside part needed rebalancing, but this is not the case. Thanks for those who wrote in. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Thu, Aug 22, 2013 at 5:19 PM, Paula Sicurello pat...@gmail.com wrote: Emily, Since it is an ultracentrifuge it needs to be rebalanced after moving. They are such picky things. -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. On Thu, Aug 22, 2013 at 10:30 AM, Emily Sours talulahg...@gmail.comwrote: I don't know how many of you use ultracentrifuges, but when you move them, do they need to be recalibrated? I can't find anything on it, but I don't want to break it because I moved it around too much. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] centrifuge?
I don't know how many of you use ultracentrifuges, but when you move them, do they need to be recalibrated? I can't find anything on it, but I don't want to break it because I moved it around too much. Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] centrifuge
Hello, I am posting this for a group of veterinary students that volunteer for the local Greyhound Rescue chapter. Just a faint hope. They are looking for a donated centrifuge (benchtop, 5 ml tubes). It is to test the blood samples of rescued dogs to check for parasites before shipping the dogs across provincial/state/national borders. This use would not be restricted to Greyhounds, but also for any other volunteer work that the many veterinary student groups do here; , treating/spay/neuter feral cats, going up North and treating sled dogs, Wild Life rescue etc. We have very active caring students here. They have canvassed the vet college here , university campus etc. but no one has a small unused centrifuge. Thanks, Margaret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
