Re: [Histonet] clostridium and bacterial spores in tissue sections
I used to flame tissue sections regularly when doing a ZN for TB...a wisp of cotton wool held in forceps, dipped in alcohol and lit with a match. A few quick passes under the slide and bob's yr uncle. worked fine, until the day came that I ignited some acetone fumes in the sink and fireballed my eyebrows off! Gentle heating in a incubator also works On Sat, Jan 14, 2012 at 8:34 PM, Rene J Buesa rjbu...@yahoo.com wrote: When I was in pre-medical (59 years ago, imagine!) we used the same stain you are referring to that involved Ziehl-Nielsen and light green as counter stain with heat, so much heat, that a flame has to be applied to dry the smears. In Peter Gray's book there are about 40 methods described,none for tissues. I am not sure a tissue section will survive, but, why not, just try it, and let us all know. René J. --- On Sat, 1/14/12, Elizabeth Chlipala l...@premierlab.com wrote: From: Elizabeth Chlipala l...@premierlab.com Subject: [Histonet] clostridium and bacterial spores in tissue sections To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Saturday, January 14, 2012, 12:58 PM Hello everyone I need a bit of help. I have to stain for clostridium and bacterial spores in tissue sections. I have come across a few references to staining these on smears but not on tissue sections. I did find one reference that used Ziehl-Neelsen to stain terminal spores from clostridium tetani. Does anyone out there know if the stain they use in microbiology for smears will work on tissue sections? Any advice would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.comhttp://www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
re: [Histonet] clostridium and bacterial spores in tissue sections
Hi Liz, I'm not sure why a good old Gram (or Brown and Brenn) wouldn't work for your sections. I have visualized clostridia with such stains in the past, and the terminal spores were easily visible. __ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Québec, Canada J7H 1N8 tel: 450-435-9482 ext.247 fax: 450-435-4795 jm.lapoi...@accellab.com --- On Sat, 1/14/12, Elizabeth Chlipala l...@premierlab.com wrote: From: Elizabeth Chlipala l...@premierlab.com Subject: [Histonet] clostridium and bacterial spores in tissue sections To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Saturday, January 14, 2012, 12:58 PM Hello everyone I need a bit of help. I have to stain for clostridium and bacterial spores in tissue sections. I have come across a few references to staining these on smears but not on tissue sections. I did find one reference that used Ziehl-Neelsen to stain terminal spores from clostridium tetani. Does anyone out there know if the stain they use in microbiology for smears will work on tissue sections? Any advice would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.comhttp://www.premierlab.com ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] clostridium and bacterial spores in tissue sections
Hi Liz: I have used IHC to identify Clostridia on formalin-fixed tissue. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Elizabeth Chlipala l...@premierlab.com 1/14/2012 12:58 PM Hello everyone I need a bit of help. I have to stain for clostridium and bacterial spores in tissue sections. I have come across a few references to staining these on smears but not on tissue sections. I did find one reference that used Ziehl-Neelsen to stain terminal spores from clostridium tetani. Does anyone out there know if the stain they use in microbiology for smears will work on tissue sections? Any advice would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.comhttp://www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] clostridium and bacterial spores in tissue sections
Great thanks, I think I'll just try a gram stain to start off with. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean-Martin Lapointe Sent: Monday, January 16, 2012 6:21 AM To: histonet@lists.utsouthwestern.edu Subject: re: [Histonet] clostridium and bacterial spores in tissue sections Hi Liz, I'm not sure why a good old Gram (or Brown and Brenn) wouldn't work for your sections. I have visualized clostridia with such stains in the past, and the terminal spores were easily visible. __ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Québec, Canada J7H 1N8 tel: 450-435-9482 ext.247 fax: 450-435-4795 jm.lapoi...@accellab.com --- On Sat, 1/14/12, Elizabeth Chlipala l...@premierlab.com wrote: From: Elizabeth Chlipala l...@premierlab.com Subject: [Histonet] clostridium and bacterial spores in tissue sections To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Saturday, January 14, 2012, 12:58 PM Hello everyone I need a bit of help. I have to stain for clostridium and bacterial spores in tissue sections. I have come across a few references to staining these on smears but not on tissue sections. I did find one reference that used Ziehl-Neelsen to stain terminal spores from clostridium tetani. Does anyone out there know if the stain they use in microbiology for smears will work on tissue sections? Any advice would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.comhttp://www.premierlab.com ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] clostridium and bacterial spores in tissue sections
When I was in pre-medical (59 years ago, imagine!) we used the same stain you are referring to that involved Ziehl-Nielsen and light green as counter stain with heat, so much heat, that a flame has to be applied to dry the smears. In Peter Gray's book there are about 40 methods described,none for tissues. I am not sure a tissue section will survive, but, why not, just try it, and let us all know. René J. --- On Sat, 1/14/12, Elizabeth Chlipala l...@premierlab.com wrote: From: Elizabeth Chlipala l...@premierlab.com Subject: [Histonet] clostridium and bacterial spores in tissue sections To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Saturday, January 14, 2012, 12:58 PM Hello everyone I need a bit of help. I have to stain for clostridium and bacterial spores in tissue sections. I have come across a few references to staining these on smears but not on tissue sections. I did find one reference that used Ziehl-Neelsen to stain terminal spores from clostridium tetani. Does anyone out there know if the stain they use in microbiology for smears will work on tissue sections? Any advice would be appreciated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.comhttp://www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
