Try 1-2% Ethyl green in acetate buffer (pH4) - will give you green nuclei (of
course)
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Tuesday, 15 November 2011 3:59 AM
To: Histonet
Subject: [Histonet] counterstain for fast red
Hi Everyone,
We are currently running some alk phos staining protocols with a fast red
chromogen. My pathologist would like to use something other than Hematoxylin
as a counterstain. What does everyone recommend?
Thanks,
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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