RE: [Histonet] cutting bone with metal
Bone with metal implants will require ground sections prepared from methyl methacrylate embedded samples, microtome sections even using tungsten carbide knives and MMA embedding cannot usually be done in my experience. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pru...@ihctech.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Friday, October 26, 2012 3:16 AM To: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: Re: [Histonet] cutting bone with metal Talk with Jack Ratliff, Chair of the NSH Hard Tissue Committee. Jack L. Ratliff 615-236-4901 ratliffj...@gmail.com The answer is Yes, histologic sections can be made, but need plastic resins (methyl methracylate or glycol methacrylate or something similar) and special microtomes and knives. If the researcher's lab doesn't do this technique, Jack can let him know who does, and the tissue can be sent out to the specialty lab. Paraffin blocks on regular histology microtomes won't cut it - literally and figuratively. Peggy Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are my own, and do not reflect on Beaumont Hospital. -Original Message- From: Jennifer MacDonald Sent: Thursday, October 25, 2012 11:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting bone with metal I have been asked the following question. I do not have an answer and was hoping someone in the Histonet community did. Thanks. There is a researcher who is doing orthopedic procedures on broken rat tibias. The researcher is repairing the tibias with metal rods or plates.not sure which (and the doctor isn't sure what kind of metal either). The researcher wants to know if it is possible to make histologic sections of the repaired tibias with the metal intact ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cutting bone with metal
Talk with Jack Ratliff, Chair of the NSH Hard Tissue Committee. Jack L. Ratliff 615-236-4901 ratliffj...@gmail.com The answer is Yes, histologic sections can be made, but need plastic resins (methyl methracylate or glycol methacrylate or something similar) and special microtomes and knives. If the researcher's lab doesn't do this technique, Jack can let him know who does, and the tissue can be sent out to the specialty lab. Paraffin blocks on regular histology microtomes won't cut it - literally and figuratively. Peggy Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are my own, and do not reflect on Beaumont Hospital. -Original Message- From: Jennifer MacDonald Sent: Thursday, October 25, 2012 11:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting bone with metal I have been asked the following question. I do not have an answer and was hoping someone in the Histonet community did. Thanks. There is a researcher who is doing orthopedic procedures on broken rat tibias. The researcher is repairing the tibias with metal rods or plates…not sure which (and the doctor isn't sure what kind of metal either). The researcher wants to know if it is possible to make histologic sections of the repaired tibias with the metal intact ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cutting bone with metal
Thank you Peggy for the kind intro! Both Peggy and Andrew are correct that it is possible to perform histology on this tissue and with the metal remaining intact with the specimen. As they both have stated, resin embedding is required to accomplish this, along with some form of saw sectioning using a diamond studded material like a wire saw, disc wheel, or band saw blade. I have even witnessed and personally used a non-contact laser method to cut sections from specimens containing metallic implants, but that is another discussion in itself! Please feel free to have your researcher contact me directly (see contact info below) Jennifer and I will make sure they get the information they might need to move forward with their study. Best Regards, Jack ratliffj...@gmail.com 615-236-4901 (o) 317-281-1975 (c) On Oct 26, 2012, at 4:16 AM, Lee Peggy Wenk lpw...@sbcglobal.net wrote: Talk with Jack Ratliff, Chair of the NSH Hard Tissue Committee. Jack L. Ratliff 615-236-4901 ratliffj...@gmail.com The answer is Yes, histologic sections can be made, but need plastic resins (methyl methracylate or glycol methacrylate or something similar) and special microtomes and knives. If the researcher's lab doesn't do this technique, Jack can let him know who does, and the tissue can be sent out to the specialty lab. Paraffin blocks on regular histology microtomes won't cut it - literally and figuratively. Peggy Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 The opinions expressed are my own, and do not reflect on Beaumont Hospital. -Original Message- From: Jennifer MacDonald Sent: Thursday, October 25, 2012 11:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting bone with metal I have been asked the following question. I do not have an answer and was hoping someone in the Histonet community did. Thanks. There is a researcher who is doing orthopedic procedures on broken rat tibias. The researcher is repairing the tibias with metal rods or plates…not sure which (and the doctor isn't sure what kind of metal either). The researcher wants to know if it is possible to make histologic sections of the repaired tibias with the metal intact ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cutting bone with metal
I have been asked the following question. I do not have an answer and was hoping someone in the Histonet community did. Thanks. There is a researcher who is doing orthopedic procedures on broken rat tibias. The researcher is repairing the tibias with metal rods or plates…not sure which (and the doctor isn't sure what kind of metal either). The researcher wants to know if it is possible to make histologic sections of the repaired tibias with the metal intact ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
