Dear histonetters!

I would be happy about some input about decalcification protocols with EDTA
of trephine bonemarrow biopsies.

recommended duration of fixation?

recommended duration of decalcification?

strategies for speed-up of decal?

recommended EDTA-solution formula?

 

Hopefully some experienced histotechs can share their knowledge with me.

thanks in advance

Gudrun

 

 

 

 

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