We have excellent sucess in decalcifying the bone prior to processing. We make
sure the core has been in fixative for 2 hrs prior to the 20 mins in the decal
solution (DeCal STAT). It is then rinced in water and placed in line for
standard processing.
Matt Lunetta HT (ASCP)
Longmont United Hospital
Message: 3
Date: Sat, 15 Jan 2011 19:16:14 -0500
From: Richard Cartun
Subject: Re: [Histonet] RE: decalcifying bone marrows after processing
To: ,,
Message-ID: 4d31f280027700020...@gwmail1.harthosp.org
Content-Type: text/plain; charset=US-ASCII
Hi Becky:
How long is the specimen in formalin before you put it in Cal Rite decal?
Thanks.
Richard
Richard W. Cartun, Ph.D.
Director, Histology Immunopathology
Director, Biospecimens
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax
Garrison, Becky 01/14/11 5:44 PM
We process bone marrow biopsies the same day received. We use a combination
decal/ fixative solution (Cal Rite decal) with good results. We keep in this
solution a minimum of 3 hours before moving to
tissue processor where first 2 solutions are a total of 3 hours in
formalin. Although most bone marrow biopsies are received by 2:00 - 3:00pm;
we've had some received as late as 4:00pm with good results.
Our clinicians place the bone marrow core in pre-filled formalin at the
point of collection, also.
The Cal Rite is a combination of formaldehyde, formic acid and methanol.
Becky Garrison
Pathology Supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-244-6237, phone
904-244-4290, fax
904-393-3194, pager
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Powers, Kerry
Sent: Friday, January 14, 2011 4:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decalcifying bone marrows after processing
I was wondering if anyone has any experience with, or is it even possible to,
decalcify bone marrows after they are processed. Our pathologist would like to
be able to process bone marrows the same day we receive them, but most of the
time there just isn't enough time to allow for proper fixation and then proper
decalcification. She asked if we could process them and then decalcify and I
have yet to find an answer to this question. Please help!!
Thank you,
Kerry Powers
Comanche Country Memorial Hospital
Department of Pathology
3401 W Gore, Lawton OK 73505
(580) 355-8699 ext. 3359
Fax: (580) 585-5462
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