Acid-alcohol should do the trick if you don't mind decalcifying the bone at the
same time. If the basic fuchsine is for showing micro-cracks, decalcification
might not be a good idea, so how about domestic bleach (sodium hypochlorite)?
John Kiernan
Anatomy, UWO
London, Canada
= = =
- Original Message -
From: John Baker bak...@umich.edu
Date: Wednesday, August 19, 2009 14:09
Subject: [Histonet] destaining bone
To: Histonet@lists.utsouthwestern.edu
Hello All, I hope someone can assist us with a protocol
for
destaining some mouse ulna. A student stained his bones
for 12 hours
in 1% basic fuchsin in graded ethyl alcohol from 80 % , 90 % and
then
100% as a prestain then into 100 % ethyl alcohol to destain some
prior
to pmma plastic embedding and sectioning to look at microdamage
in the
cortical bone. The bones are over stained and if possible
we would
like to destain the bone and continue on without getting
more
specimens. Any suggestions are welcome. Thank you in
advance. John
Baker and Mathieu Davis
John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200
734-936-1635
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
