Re: [Histonet] Diastase
Why buy? Just think of squeezing a chunk of lemon over your helping of haddock and drool into a small beaker. Remove bubbles by wiping the surface of the collected liquid with the edge of a piece of filter paper. Incubate for 30 minutes at 37C, just the same as for 0.1% malt diastase. Saliva also contains RNase, but this doesn't matter because RNA is not stained by PAS or by other methods for staining glycogen. For a cheap source of RNase you can heat the collected saliva (80C for 10 minutes) to inactivate diastase and any other enzymes. Cool and centrifuge. Use the supernatant at 37C for 1 hour. Some references. Bradbury S (1956) Human saliva as a convenient source of ribonuclease. Quart. J. Microsc. Sci. 97: 323-327. (Free PDF available at https://jcs.biologists.org/content/s3-97/39/323.short). Brown, GG (1978) An Introduction to Histotechnology. New York: Appleton-Century-Crofts. pp. 302 (diastase) & 292 (ribonuclease). Drury RAB, Wallington EA (1967) Carleton's Histological Technique. 4th ed. Oxford University Press, Oxford. pp.163-164 (ribonuclease) & 208 (diastase). John Kiernan Dept of Anatomy & Cell Biology University of Western Ontario, London, Canada = = = From: Paula via Histonet Sent: 26 November 2019 12:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Diastase Hello, We've been using STATLabs Diastase for our PAS with diastase digestion but they have a backorder until January. Can anyone recommend an alternative from other vendors? Thank you in advance, Paula ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Diastase
Hello, We've been using STATLabs Diastase for our PAS with diastase digestion but they have a backorder until January. Can anyone recommend an alternative from other vendors? Thank you in advance, Paula ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] diastase
Porcine amylase has worked for us (of course, so has the spit!) Janet From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com [sgoe...@mirnarx.com] Sent: Wednesday, March 09, 2011 9:56 AM To: dmcc...@ckha.on.ca; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] diastase Just hack up some spit... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, March 09, 2011 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] diastase I am looking to purchase diastase to use for the PAS/DIASTASE stain. I see there are many options in the catalogues. What is recommended? diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] diastase
I am looking to purchase diastase to use for the PAS/DIASTASE stain. I see there are many options in the catalogues. What is recommended? diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] diastase
I have a long distance on-line histology student in my lab right now getting her practical experience. She was doing special stains this past week and had PAS with and without diastase. I had her do the stains with both commercially available diastase and also using saliva so she could see the difference. Nothing like the stains we use to do in the Good Ol' Days! What a difference. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, March 09, 2011 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] diastase I am looking to purchase diastase to use for the PAS/DIASTASE stain. I see there are many options in the catalogues. What is recommended? diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] diastase
Just hack up some spit... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, March 09, 2011 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] diastase I am looking to purchase diastase to use for the PAS/DIASTASE stain. I see there are many options in the catalogues. What is recommended? diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
