Do you mean to try to demonstrate if the fungi you find in a sample WAS dead or
alive before it was killed during the fixation and processing?
My take on this is the following: if a fungi dies naturally in a tissue
(either lung or nail, or whatever) that dead fungi either is decayed and lost
or its shape has to be modified in a way that it can be microscopically
distinguished by the pathologist from other alive fungi.
On the other hand you could try to use DNA or RNA staining to localize the
nuclei and decide if the structure seems to correspond to that of an alive
fungi.
I think that if you find fungal structures in abundance in a FFPE tissue they
should correspond to living fungi at the moment the FFPE process started.
René J.
From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
Sent: Thursday, October 11, 2012 7:53 AM
Subject: [Histonet] help with dead fungi
Good morning everyone,
Does anyone have a protocol they use to demonstrate dead fungi in FFPPE tissue?
Thanks!
Jeanine H. Bartlett, BS HT(ASCP), QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, NE
MS/G-32
Atlanta, Ga 30333
404-639-3590
jeanine.bartl...@cdc.hhs.gov
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