Is there an alternative to toluidine blue stain for plastic embedded tissue? I have some valuable epoxy resin embedded rat spinal cord blocks that were not well osmicated. Toluidine Blue staining of the semi-thin sections is uneven and pale in the center of the sections. Is there another stain I could try that is not dependent on good osmium penetration? I fear that alcohol dehydration and propylene oxide clearing may have demyelinated the poorly osmicated portions of the block. In that case, no stain will work. But I'm not ready to give up yet.
Thanks for any and all advice. I've been out of the lab for a while. Mollie Lange Project Manager International Center for Spinal Cord Injury Huo W. Moser Research Institute at Kennedy Krieger 707 N. Broadway Baltimore, MD 21205 443-923-9241 phone 443-923-9245 fax la...@kennedykrieger.org<mailto:la...@kennedykrieger.org> Disclaimer: The materials in this e-mail are private and may contain Protected Health Information. Please note that e-mail is not necessarily confidential or secure. Your use of e-mail constitutes your acknowledgment of these confidentiality and security limitations. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via telephone or return e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
