[Histonet] how to make crashed ice?

2010-08-07 Thread Gudrun Lang
Hi!

I think this is a rather basic question ;-), but I'm looking for practical
advice.

We are going to try the OSNA-test for sentinel nodes. The application needs
a pot with crashed ice while desintegrating the tissue with a mixer. So over
the day this should be four to six litre ice, if we have to take fresh ice
for each time, a group of sentinels has to be worked up.

 

What is a practical way to make crashed ice in the lab? 

Thanks for your answers in advance!

Gudrun

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Re: [Histonet] how to make crashed ice?

2010-08-07 Thread louise renton
Hi gudrun,

Place the ice - ice cubes works best in a plastic bag, wrap in a towel and
bash with a heavy object like a hammer. You can also use Jamie Oliver's
trick - put ice cubes in a cloth tea towel, , bring the  4 corners together,
tie them in knot, and then hit the parcel on the edge of your work surface
until the ice is the sze you need

hope this helps

On Sat, Aug 7, 2010 at 12:12 PM, Gudrun Lang gu.l...@gmx.at wrote:

 Hi!

 I think this is a rather basic question ;-), but I'm looking for practical
 advice.

 We are going to try the OSNA-test for sentinel nodes. The application needs
 a pot with crashed ice while desintegrating the tissue with a mixer. So
 over
 the day this should be four to six litre ice, if we have to take fresh ice
 for each time, a group of sentinels has to be worked up.



 What is a practical way to make crashed ice in the lab?

 Thanks for your answers in advance!

 Gudrun

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-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel  fax)
073 5574456 (emergencies only)
There are nights when the wolves are silent and only the moon howls.
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
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Re: [Histonet] how to make crashed ice?

2010-08-07 Thread koellingr


Hello, 

It is very inexpensive, a few dollars, to buy a counter-top ice crusher, that a 
professional bartender might use to crush ice for drinks.  Can be bought at 
most any store.  We crush ice every day on a daily basis to create a small tub 
of  ice we need for reagents.  Only the size of like a small kitchen food 
processor. Just add ice cubes and crush them in a few seconds without a hammer 
and the mess. 



Ray 

PhenoPath Labs 

Seattle, WA 


- Original Message - 
From: louise renton louise.ren...@gmail.com 
To: gu lang gu.l...@gmx.at, Histonet@lists.utsouthwestern.edu 
Sent: Saturday, August 7, 2010 3:24:17 AM 
Subject: Re: [Histonet] how to make crashed ice? 

Hi gudrun, 

Place the ice - ice cubes works best in a plastic bag, wrap in a towel and 
bash with a heavy object like a hammer. You can also use Jamie Oliver's 
trick - put ice cubes in a cloth tea towel, , bring the  4 corners together, 
tie them in knot, and then hit the parcel on the edge of your work surface 
until the ice is the sze you need 

hope this helps 

On Sat, Aug 7, 2010 at 12:12 PM, Gudrun Lang gu.l...@gmx.at wrote: 

 Hi! 
 
 I think this is a rather basic question ;-), but I'm looking for practical 
 advice. 
 
 We are going to try the OSNA-test for sentinel nodes. The application needs 
 a pot with crashed ice while desintegrating the tissue with a mixer. So 
 over 
 the day this should be four to six litre ice, if we have to take fresh ice 
 for each time, a group of sentinels has to be worked up. 
 
 
 
 What is a practical way to make crashed ice in the lab? 
 
 Thanks for your answers in advance! 
 
 Gudrun 
 
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 Histonet mailing list 
 Histonet@lists.utsouthwestern.edu 
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-- 
Louise Renton 
Bone Research Unit 
University of the Witwatersrand 
Johannesburg 
South Africa 
+27 11 717 2298 (tel  fax) 
073 5574456 (emergencies only) 
There are nights when the wolves are silent and only the moon howls. 
George Carlin 
No trees were killed in the sending of this message. 
However, many electrons were terribly inconvenienced. 
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