I've made my own in situ buffer (for chromosome probes) using a simple
recipe:
5ml 2xSSC pH6.8
5ml formamide (deionized)
1g dextran sulfate (DS) Sigma D6001
Warm the 2xSSC and add the DS to dissolve. Add formamide. Sterile filter.
Aliquot and store at -20 C or lower.
Hope this helps!
Regards,
Merced
--On Tuesday, February 01, 2011 12:52 PM +0200 louise renton
louise.ren...@gmail.com wrote:
Greetings, Oh mighty histonetters!
As per previous posts I am re-embarking on doing in situ hybridization. I
will be using (hopefully) DIG labelled oligo probes, and ultimately
visualizing with DAB.
My question isare there a commercially available hybrizidation
buffers? looking at the extensive list of ingredients it would be far
easies (and cheaper) to get ready made stufff
best regards
--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel fax)
073 5574456 (emergencies only)
There are nights when the wolves are silent and only the moon howls.
George Carlin
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
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