[Histonet] in-situ hybridization
This questions is for the labs that do in-situ hybridization for diagnostic purposes. Do you take slides for ISH from other labs and if so, please contact me via my email. My next question is; one of my doc's is interested in us doing ISH herein our lab but I have been putting her off with the fact that we do not need this very often, it is very expensive to start up, etc. But she is persistent. Any suggestions for starting up this procedure, including vendor preferences for supplies? I know next to nothing about ISH other than what I have read on-line. Thank you in advance Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] in-situ hybridization
Roche Diagnostics has great DIG labels, antibodies and color reaction chemicals. It is a pain to just start up, you need to buy a lot of stuff for it. Could you use someone else's set up and pay for part of the chemicals? Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Apr 19, 2011 at 12:51 PM, Bales, Candy A candy.a.ba...@uth.tmc.eduwrote: This questions is for the labs that do in-situ hybridization for diagnostic purposes. Do you take slides for ISH from other labs and if so, please contact me via my email. My next question is; one of my doc's is interested in us doing ISH herein our lab but I have been putting her off with the fact that we do not need this very often, it is very expensive to start up, etc. But she is persistent. Any suggestions for starting up this procedure, including vendor preferences for supplies? I know next to nothing about ISH other than what I have read on-line. Thank you in advance Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] in situ hybridization
Hi all, after a decade of not doing ISH, I have been asked to initiate a project in our department. needless to say i am VERY rusty. can any one suggest: - a good on-line resource to brush up my knowledge - a company that have reference materials/handbooks etc (I seem to recall Roche did this)? - A course/workshop that i can attend specifically on embryo and bone in situ? As always i thank the forum for its help best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] in situ hybridization histochemistry question about radioisotope
I have routinely seen that we obtain slightly different results (like 10-20% variance) in using 35S dATP for TdT-catalyzed tailing reactions of oligonucleotides from month to month as we use different batches (made fresh monthly) of the 35S dATP. But on a few occasions over the years we have found dramatically reduced results with a particular lot. This happened again in February, and I confirmed by comparing directly between the Feb. March lots in March that the results with the Feb. lot were half that of the March batch (of course, given the half-life of 35S, they would be expected to be down some, but not by half!). The company was good about believing me and not charging us for the March replacement batch, but they have no idea what could cause this to happen occasionally. They check the specific activity, pH, purity, etc. and run a bio-assay that is a binding assay each month, but were not set up to test the enzymatic reaction. I told them that years ago when this happened it was finally determined that the DTT concentration was unusually high and interfered with the reaction, but they discounted this possibility. I guess I should just count my blessings that things are working again now (mercifully, I was able to cling to optimism, based on past experience, that the isotope was the problem, until I could confirm this myself), but it's very frustrating that the company is not motivated to check the efficacy of the item for this particular application (which I would imagine a lot of customers use it for) each month, or to figure out what causes this to happen on occasion (so as to avoid it!)--we lose a lot of time and other expensive reagents (e.g. the TdT) each time this happens. I also don't know how to interpret it--I know that as the enzyme begins to degrade it just means that the tails are shorter, which I can compensate for with a longer film exposure, but I have no idea what's going on when the isotope causes the problem am afraid to use those batches. Does anyone else using ISH have any ideas about what causes this variability? grateful for any thoughts, Susan No virus found in this outgoing message. Checked by AVG - www.avg.com Version: 8.5.436 / Virus Database: 271.1.1/2745 - Release Date: 03/13/10 21:51:00 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
