We have the Leica linear stainer (Also sold by Avantik?) and have it set at 7 second intervals. The stain takes about 100 seconds. Slides are fixed in the Coplin jar with 95% Alcohol then loaded: The stations are in order: 1. Water wash 2. Hemotoxylin (Richard Allen 7211) 3. Hemotoxylin (Richard Allen 7211) 4. Hemotoxylin (Richard Allen 7211) 5. Water wash 6. Bluing (we use Surgipath SelecTech Blue Buffer 8 concentrate, diluted according to directions 7. Water wash 8. Eosin (1% Alcoholic) 9. 95% Alcohol 10. Absolute Alcohol 11. Absolute Alcohol 12. Absolute Alcohol 13. Xylene 14. Xylene
The slides are clean and consistent with great differentiation. The Richard Allen 7211 Hematoxylin does not require filtering and seems to last forever. I sincerely hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Routine H&E frozen section protocol (Raible, John C.) 2. Re: Routine H&E frozen section protocol (Porter, Amy) 3. FFPE PRC testing (Charles Riley) ---------------------------------------------------------------------- Message: 1 Date: Fri, 24 Apr 2020 12:10:26 +0000 From: "Raible, John C." <john.rai...@va.gov> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Routine H&E frozen section protocol Message-ID: <dm6pr09mb600818ca6aae3a60df394a6281...@dm6pr09mb6008.namprd09.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Hello Histonet, My name is John Raible, and I work at the VA medical center in Minneapolis. I am trying to find routine H&E frozen section staining protocols for an automated linear stainer. My lab recently got one, and I was asked to research staining protocols Any help you could provide would be greatly appreciated. Thank you all. Stay safe and healthy! John ------------------------------ Message: 2 Date: Fri, 24 Apr 2020 12:26:28 +0000 From: "Porter, Amy" <port...@msu.edu> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "Raible, John C." <john.rai...@va.gov> Subject: Re: [Histonet] Routine H&E frozen section protocol Message-ID: <ch2pr12mb4230567afe1b34e45a537347a4...@ch2pr12mb4230.namprd12.prod.outlook.com> Content-Type: text/plain; charset="iso-8859-1" Fix in 10% NBF - 10 minutes Rinse in running tap water - 5 minute Rinse in distilled water Gill 2 Hematoxylin - 1 1/2 minutes 1% Glacial Acetic water - 3 dips Running water - 2 minutes (we do not need bluing agent - our water takes care of it) 95% Ethanol -2 minutes 1% Eosin/Phloxine - 2 minutes 95% Ethanol - 2 minutes Remaining dehydration in absolute ethanol & xylene - 2 mintues rest of the way down ________________________________ From: Raible, John C. via Histonet <histonet@lists.utsouthwestern.edu> Sent: Friday, April 24, 2020 8:10 AM To: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Routine H&E frozen section protocol Hello Histonet, My name is John Raible, and I work at the VA medical center in Minneapolis. I am trying to find routine H&E frozen section staining protocols for an automated linear stainer. My lab recently got one, and I was asked to research staining protocols Any help you could provide would be greatly appreciated. Thank you all. Stay safe and healthy! John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!jZVhszUj4q_tZtfO2cKIo_OQbyOsjHXT6pEGAn_UXytcWKRzM703NRorymJh6A$ ------------------------------ Message: 3 Date: Fri, 24 Apr 2020 12:13:58 -0400 From: Charles Riley <cri...@dpspa.com> To: Histo List <histonet@lists.utsouthwestern.edu> Subject: [Histonet] FFPE PRC testing Message-ID: <CAAQhB13qr=1noiktrh07qm0ot0qb3ovslajw97hstqkjkyz...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" What platform does everyone use to carry out FFPE PCR testing? Seeking to bring in as many genetic mutation testing options as possible (BRAF, KRAS, NRAS...etc.) -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 197, Issue 22 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet