Your decal time should be according to your mice age. Four weeks old mouse normally need four changes of EDTA in two weeks time. I think your process time is a bit too long. If your four weeks old mouse tibia, 13 hours from 70% ethanol to last infiltration paraffin should be fine. Your shrinkage is because 1. temperature too high?; 2. too long in xylene? hope this help. Dorothy Hu
Hi does any one doing mice tibia histology, we use to fix in formalin for 24 hours and transfer in 2and half weeks in 0.5M EDTA three change and process for 24 hours long protocol in automatic processor. But I am facing problem with bone marrow shrinkage. If any one have idea for decalcification timing and solution can resolved this problem and keep bone marrow intact with bone. Thank you in advance. Regards, Shruti _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet