[Histonet] micro wave processors
I am in a mirotomy class, and we had a discussion about mircorwave processing. We need an accurate temperature. Freida states it should be at 84 degrees however we think she is incorrect. please respond asap. thank you leticia alvarez ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] micro wave processors
You will always have a recommended protocol. It is up to us as HT/HTL to determine what exactly will work. That is where TROUBLE SHOOTING falls in. As I posted earlier, I use a certain type of microwave processor and because the recommended temps did not work on the type of tissue I was processing. I had to trouble shoot for what would work. Again, my paraffin temps do not exceed 67C. THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 909-841-9761. From: jose and leticia alvarez alvarez092...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 8, 2011 8:05 AM Subject: [Histonet] micro wave processors I am in a mirotomy class, and we had a discussion about mircorwave processing. We need an accurate temperature. Freida states it should be at 84 degrees however we think she is incorrect. please respond asap. thank you leticia alvarez ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] micro wave processors
From my research I have found that the molecular water (polar) content and insulating fat content of the tissue types are added considerations. If you look at the physics and how microwaves penetrate and generate heat, this really helps in customizing schedules for different tissues and using its properties and effects to best advantage. The goods news is that the MW processors have more developed software that allows a wide manipulation ( in my experience) for processing protocols. Still, as you mentioned troubleshooting is as always required. Being newer technology, there are some implementation hurdles, but I have seen it work sucessfully for at least a large volume of various tissue types, signifigantly decreasing turn around times and leveling out workflow that allows staffing to be adjusted in favorable ways. Some labs I have worked at always seem to have certain tissues though that they have not been able to optimize a suitable protocol for processing with microwave assistance so far. Still in the refinement and development stages in many cases.Joelle Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver Date: Tue, 8 Nov 2011 09:30:13 -0800 From: nelsonr...@verizon.net To: alvarez092...@hotmail.com Subject: Re: [Histonet] micro wave processors CC: Histonet@lists.utsouthwestern.edu You will always have a recommended protocol. It is up to us as HT/HTL to determine what exactly will work. That is where TROUBLE SHOOTING falls in. As I posted earlier, I use a certain type of microwave processor and because the recommended temps did not work on the type of tissue I was processing. I had to trouble shoot for what would work. Again, my paraffin temps do not exceed 67C. THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 909-841-9761. From: jose and leticia alvarez alvarez092...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 8, 2011 8:05 AM Subject: [Histonet] micro wave processors I am in a mirotomy class, and we had a discussion about mircorwave processing. We need an accurate temperature. Freida states it should be at 84 degrees however we think she is incorrect. please respond asap. thank you leticia alvarez ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] micro wave processors
67 is too hot for vacuum-pressure assisted conventional processing. MW processors use up to 84 to evaporate the IPA, but the time periods are often as little as 5 minutes. Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver Date: Tue, 8 Nov 2011 09:30:13 -0800 From: nelsonr...@verizon.net To: alvarez092...@hotmail.com Subject: Re: [Histonet] micro wave processors CC: Histonet@lists.utsouthwestern.edu You will always have a recommended protocol. It is up to us as HT/HTL to determine what exactly will work. That is where TROUBLE SHOOTING falls in. As I posted earlier, I use a certain type of microwave processor and because the recommended temps did not work on the type of tissue I was processing. I had to trouble shoot for what would work. Again, my paraffin temps do not exceed 67C. THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 909-841-9761. From: jose and leticia alvarez alvarez092...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 8, 2011 8:05 AM Subject: [Histonet] micro wave processors I am in a mirotomy class, and we had a discussion about mircorwave processing. We need an accurate temperature. Freida states it should be at 84 degrees however we think she is incorrect. please respond asap. thank you leticia alvarez ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] micro wave processors
I should add, believe includes placental tissue or POC Sent from my Verizon Wireless BlackBerry -Original Message- From: joelle weaver joellewea...@hotmail.com Date: Tue, 8 Nov 2011 17:42:41 To: nelsonr...@verizon.net; histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] micro wave processors From my research I have found that the molecular water (polar) content and insulating fat content of the tissue types are added considerations. If you look at the physics and how microwaves penetrate and generate heat, this really helps in customizing schedules for different tissues and using its properties and effects to best advantage. The goods news is that the MW processors have more developed software that allows a wide manipulation ( in my experience) for processing protocols. Still, as you mentioned troubleshooting is as always required. Being newer technology, there are some implementation hurdles, but I have seen it work sucessfully for at least a large volume of various tissue types, signifigantly decreasing turn around times and leveling out workflow that allows staffing to be adjusted in favorable ways. Some labs I have worked at always seem to have certain tissues though that they have not been able to optimize a suitable protocol for processing with microwave assistance so far. Still in the refinement and development stages in many cases.Joelle Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver Date: Tue, 8 Nov 2011 09:30:13 -0800 From: nelsonr...@verizon.net To: alvarez092...@hotmail.com Subject: Re: [Histonet] micro wave processors CC: Histonet@lists.utsouthwestern.edu You will always have a recommended protocol. It is up to us as HT/HTL to determine what exactly will work. That is where TROUBLE SHOOTING falls in. As I posted earlier, I use a certain type of microwave processor and because the recommended temps did not work on the type of tissue I was processing. I had to trouble shoot for what would work. Again, my paraffin temps do not exceed 67C. THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 909-841-9761. From: jose and leticia alvarez alvarez092...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 8, 2011 8:05 AM Subject: [Histonet] micro wave processors I am in a mirotomy class, and we had a discussion about mircorwave processing. We need an accurate temperature. Freida states it should be at 84 degrees however we think she is incorrect. please respond asap. thank you leticia alvarez ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] micro wave processors
I should add, believe includes placental tissue or POC Sent from my Verizon Wireless BlackBerry -Original Message- From: joelle weaver joellewea...@hotmail.com Date: Tue, 8 Nov 2011 17:42:41 To: nelsonr...@verizon.net; histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] micro wave processors From my research I have found that the molecular water (polar) content and insulating fat content of the tissue types are added considerations. If you look at the physics and how microwaves penetrate and generate heat, this really helps in customizing schedules for different tissues and using its properties and effects to best advantage. The goods news is that the MW processors have more developed software that allows a wide manipulation ( in my experience) for processing protocols. Still, as you mentioned troubleshooting is as always required. Being newer technology, there are some implementation hurdles, but I have seen it work sucessfully for at least a large volume of various tissue types, signifigantly decreasing turn around times and leveling out workflow that allows staffing to be adjusted in favorable ways. Some labs I have worked at always seem to have certain tissues though that they have not been able to optimize a suitable protocol for processing with microwave assistance so far. Still in the refinement and development stages in many cases.Joelle Joelle Weaver MAOM, BA, (HTL) ASCP http://www.linkedin.com/in/joelleweaver Date: Tue, 8 Nov 2011 09:30:13 -0800 From: nelsonr...@verizon.net To: alvarez092...@hotmail.com Subject: Re: [Histonet] micro wave processors CC: Histonet@lists.utsouthwestern.edu You will always have a recommended protocol. It is up to us as HT/HTL to determine what exactly will work. That is where TROUBLE SHOOTING falls in. As I posted earlier, I use a certain type of microwave processor and because the recommended temps did not work on the type of tissue I was processing. I had to trouble shoot for what would work. Again, my paraffin temps do not exceed 67C. THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 909-841-9761. From: jose and leticia alvarez alvarez092...@hotmail.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 8, 2011 8:05 AM Subject: [Histonet] micro wave processors I am in a mirotomy class, and we had a discussion about mircorwave processing. We need an accurate temperature. Freida states it should be at 84 degrees however we think she is incorrect. please respond asap. thank you leticia alvarez ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
