[Histonet] Osteoid fluorescence staining
Hey Histonetters, Can anybody tell me a fluorescence stain for osteoid. I need to measure the osteoid volume around/at the surface of bone lacunae with confocal laser scanning microscopy. I heard about basic fuchsin but there the lacunae voids are also stained so i guess it's hard then to distinguish between the void and the osteoid. Thanks for your help. Michael === Michael Kerschnitzki Biomaterials Department Max Planck Institute of Colloids and Interfaces Postal address: MPI KGF Golm, D - 14424 Potsdam, Germany Physical address: Am Mühlenberg 1, D - 14476 Golm Germany Telephone: +49 (331) 567 - 9464 Fax: +49 (331) 567 - 9402 Email: kerschnit...@mpikg.mpg.de === ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] osteoid
Thnaks for all the replies I got on this subject - they have clarified my thoughts somewhat -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Osteoid identifiction
Hi Louise, Yes, you are right best way to quantify the osteoid is plastic embedding (MMA) but recently I saw a paper in Stain Technology the title is Silver Staining of bone prior to decalcification for quantitative determination of osteoid in sections by E.J. Tripp and E.H. MaoKay, dept of pathology, Frenchay hospital, Bristol, England. Try that paper If you don't have excess to the paper please do let me know I will send you my copy. Good Luck! Shakun -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of louise renton Sent: Thursday, November 20, 2008 11:48 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Osteoid identifiction Hi al, I have received a sample of ?bone where the clinician wants to identfy osteoid. According to my knowledge (now perhaps a little daed) the traditional processing method is plastic embedding of some sort. Is this still the case or are there methods to use standars paraffin processing? Best regards-- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Osteoid identifiction
Hi al, I have received a sample of ?bone where the clinician wants to identfy osteoid. According to my knowledge (now perhaps a little daed) the traditional processing method is plastic embedding of some sort. Is this still the case or are there methods to use standars paraffin processing? Best regards-- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
