We routinely use the following
Hew-Shue (1991) has described a useful pH indicator for working neutral
buffered formalin solutions. Bromocresol purple (CAS: 115-40-2) , when added to
formalin solutions, serves as an indicator of pH as well as, from a safety
aspect, labelling the solution as formalin making redundant the dangerous
"smell" test. At an acidic pH (5.2) the indicator colour is yellow whereas at
pH 6.8, the colour is purple.
A saturated solution of the dye (about 0.2g/100mls) is prepared and 2 to 4
drops are added to 10 litres of neutral buffered formalin.
Hew-Shue N (1991) "Bromcresol Purple as a Colored Marker and pH Indicator for
Ten Percent Neutral Buffered Forrnalin" J Histotechnol 14(4):257-260.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-Original Message-
From: Baldwin, Kathy via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, 7 June 2018 4:09 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] pH of formalin
Hi Histonetters
Is anyone out there monitoring their Ph of the formalin they use? If do how
often?
Thanks
S Kathy Baldwin ASCP, SCT
Pathology and Cytology Manager
Ph 812-996-0210
Fax 812-996-0232
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