[Histonet] problem with subbed slides

2010-02-10 Thread Hisham Mohammed
dear histonetters

following gelatin subbing of big glass slides we observed numerous white
patches in almost the entire batch of slides subbed.

what could be the remedy to remove these white patches.

waiting for a practical solution.

with regards

hisham mohammed
senior research centre
national brain research centre
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Re: [Histonet] problem with subbed slides

2010-02-10 Thread louise renton
Just for interest's sake could this be some fungal growth? Try staining a
slide with H/E and look under the microscope.

how to fix? perhaps acid digestion and resubbing?



On Wed, Feb 10, 2010 at 8:15 PM, Hisham Mohammed hisham@gmail.comwrote:

 dear histonetters

 following gelatin subbing of big glass slides we observed numerous white
 patches in almost the entire batch of slides subbed.

 what could be the remedy to remove these white patches.

 waiting for a practical solution.

 with regards

 hisham mohammed
 senior research centre
 national brain research centre
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet




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