Re: [Histonet] processor died overnight

2017-04-19 Thread Tony Henwood (SCHN) via Histonet 
Yep start at last alcohol in other processor, xylene & wax as usual.
If the tissues have remained covered in alcohol, you should not have a problem

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: Lauren Sweeney via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, 19 April 2017 10:44 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] processor died overnight

Hello Histoworld,

I came in this morning to find that the processor died halfway through process 
last night. The tissues are in 100% ETOH exactly half point. We do have a back- 
up processor. In your professional experiences, would these tissues be 
salvageable? Could I create a new program on the backup processor that finishes 
the process from that point and transfer the tissues over?

Thanks.


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Re: [Histonet] processor died overnight

2017-04-19 Thread Mayer,Toysha N via Histonet 
To help this you could place the cassettes in the other processor in the last 
100% for about 5 min, just to freshen them up.  Then proceed with the remainder 
of the process as usual.  

FYI, this will be one of our discussion questions this year in our HTL program.

T
Toysha N. Mayer D.H.Sc., MBA, HT(ASCP)
Instructor/Education Coordinator
HTL Program
MD Anderson School of Health Professions
713.563.3481
tnma...@mdanderson.org





Hello Histoworld,

I came in this morning to find that the processor died halfway through process 
last night. The tissues are in 100% ETOH exactly half point. We do have a back- 
up processor. In your professional experiences, would these tissues be 
salvageable? Could I create a new program on the backup processor that finishes 
the process from that point and transfer the tissues over?

Thanks.






--

Message: 5
Date: Wed, 19 Apr 2017 13:41:47 + (UTC)
From: Rene J Buesa <rjbu...@yahoo.com>
To: Lauren Sweeney <lmari...@uga.edu>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] processor died overnight
Message-ID: <174147213.3806623.1492609307...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

In 100% EthOL the tissues are completely "salvaged" and you can prepare the 
program to continue the steps until melted paraffin.If there are delicate 
tissue perhaps they will be "over-dried" but that is easily "compensated" 
during microtomy.Ren? 

On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet 
<histonet@lists.utsouthwestern.edu> wrote:
 

 Hello Histoworld,

I came in this morning to find that the processor died halfway through process 
last night. The tissues are in 100% ETOH exactly half point. We do have a back- 
up processor. In your professional experiences, would these tissues be 
salvageable? Could I create a new program on the backup processor that finishes 
the process from that point and transfer the tissues over?

Thanks.


___
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--

Message: 6
Date: Wed, 19 Apr 2017 16:11:51 +
From: "Terri  Braud" <tbr...@holyredeemer.com>
To: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] IF vs IHC another reason
Message-ID:
<48E053DDF6CE074DB6A7414BA05403F8130EAF@HRHEX03-HOS.holyredeemer.local>

Content-Type: text/plain; charset="us-ascii"


Another reason that IHC is used instead of IF is with IHC, one preserves the 
ability to see tissue/cell morphology through Light Microscopy at the same time 
as the visual IHC label.  Morphology is difficult to see with IF, with the 
exception of the fluorescein labeled area.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
-Original Message-
From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, 13 April 2017 11:10 PM
Hello!
I just need a help with a simple question...Is anyone can explain me what is 
the purpose between performing immunohistochemistry and Immunofluorescence?
Thanks  :)
Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: blanca.lo...@utsouthwestern.edu





--

Message: 7
Date: Wed, 19 Apr 2017 09:17:24 -0700
From: Caroline Miller <mi...@3scan.com>
To: Rene J Buesa <rjbu...@yahoo.com>
Cc: Lauren Sweeney <lmari...@uga.edu>,
"histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] processor died overnight
Message-ID: <6156911d-ede8-4c77-9c6d-36ba64baf...@3scan.com>
Content-Type: text/plain;   charset=utf-8

Yes, totally +1 to Rene, they should be fine. 
(That has totally happened to me too)!

Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297

> On Apr 19, 2017, at 6:41 AM, Rene J Buesa via Histonet 
> <histonet@lists.utsouthwestern.edu> wrote:
> 
> In 100% EthOL the tissues are completely "salvaged" and you can prepare the 
> program to continue the steps until melted paraffin.If there are delicate 
> tissue perhaps they will be "over-dried" but that is easily "compensated" 
> during microtomy.Ren? 
> 
>On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet 
> <histonet@lists.utsouthwestern.edu> wrote:
> 
> 
> Hello Histoworld,
> 
> I came in this morning to find that the processor died half

Re: [Histonet] processor died overnight

2017-04-19 Thread McNabola, Angela via Histonet 
It has happened to us as well.  I'm not sure where you work, but our processors 
have alarms that go to security.  So I have gotten a call on off hours to come 
in and fix the problem or try to trouble shoot.  Some of the newer processors 
can be linked to your phone, etc.

-Original Message-
From: Caroline Miller via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, April 19, 2017 12:17 PM
To: Rene J Buesa
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] processor died overnight

Yes, totally +1 to Rene, they should be fine.
(That has totally happened to me too)!

Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297

> On Apr 19, 2017, at 6:41 AM, Rene J Buesa via Histonet 
> <histonet@lists.utsouthwestern.edu> wrote:
>
> In 100% EthOL the tissues are completely "salvaged" and you can prepare the 
> program to continue the steps until melted paraffin.If there are delicate 
> tissue perhaps they will be "over-dried" but that is easily "compensated" 
> during microtomy.René
>
>On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet 
> <histonet@lists.utsouthwestern.edu> wrote:
>
>
> Hello Histoworld,
>
> I came in this morning to find that the processor died halfway through 
> process last night. The tissues are in 100% ETOH exactly half point. We do 
> have a back- up processor. In your professional experiences, would these 
> tissues be salvageable? Could I create a new program on the backup processor 
> that finishes the process from that point and transfer the tissues over?
>
> Thanks.
>
>
> ___
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>
>
>
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Re: [Histonet] processor died overnight

2017-04-19 Thread Caroline Miller via Histonet 
Yes, totally +1 to Rene, they should be fine. 
(That has totally happened to me too)!

Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297

> On Apr 19, 2017, at 6:41 AM, Rene J Buesa via Histonet 
>  wrote:
> 
> In 100% EthOL the tissues are completely "salvaged" and you can prepare the 
> program to continue the steps until melted paraffin.If there are delicate 
> tissue perhaps they will be "over-dried" but that is easily "compensated" 
> during microtomy.René 
> 
>On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet 
>  wrote:
> 
> 
> Hello Histoworld,
> 
> I came in this morning to find that the processor died halfway through 
> process last night. The tissues are in 100% ETOH exactly half point. We do 
> have a back- up processor. In your professional experiences, would these 
> tissues be salvageable? Could I create a new program on the backup processor 
> that finishes the process from that point and transfer the tissues over?
> 
> Thanks.
> 
> 
> ___
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> Histonet@lists.utsouthwestern.edu
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> 
> 
> 
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Re: [Histonet] processor died overnight

2017-04-19 Thread Rene J Buesa via Histonet 
In 100% EthOL the tissues are completely "salvaged" and you can prepare the 
program to continue the steps until melted paraffin.If there are delicate 
tissue perhaps they will be "over-dried" but that is easily "compensated" 
during microtomy.René 

On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet 
 wrote:
 

 Hello Histoworld,

I came in this morning to find that the processor died halfway through process 
last night. The tissues are in 100% ETOH exactly half point. We do have a back- 
up processor. In your professional experiences, would these tissues be 
salvageable? Could I create a new program on the backup processor that finishes 
the process from that point and transfer the tissues over?

Thanks.


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[Histonet] processor died overnight

2017-04-19 Thread Lauren Sweeney via Histonet 
Hello Histoworld,

I came in this morning to find that the processor died halfway through process 
last night. The tissues are in 100% ETOH exactly half point. We do have a back- 
up processor. In your professional experiences, would these tissues be 
salvageable? Could I create a new program on the backup processor that finishes 
the process from that point and transfer the tissues over?

Thanks.


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