You may have to call a tech at thermos to ask...... It still sounds like it it's the microtome as we know they cut. Join Histonet or the NSH facebook page and you can ask there as well. I may just be missing something obvious. histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> for histonet. It is a forum. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu>
From: Elizabeth Jane Lux Sent: Friday, April 21, 2017 10:04 AM To: Bernice Frederick Subject: Re: sectioning issues? Thanks so much for your help! Frustratingly having issues when I move into sectioning. Ribbons look square at trim mode (20u) but when I try to get my sections, I'm getting inconsistent slicing, almost like it's compressing it at times. Or I guess not advancing the specimen correctly? Perhaps the arm needs maintenance? Oh, and I was completely wrong on the model, it's a Thermo Finesse ME+ Liz ________________________________ From: Elizabeth Jane Lux Sent: Friday, April 21, 2017 8:58 AM To: Bernice Frederick Subject: Re: sectioning issues? Awesome! I'll bring the block(s) over now, thanks! Elizabeth Lux Research Tech 3 Feinberg Cardiovascular Research Institute Northwestern University Lurie Building, 10-220 312.503.6368 lab phone 312.503.0219 fax ________________________________ From: Bernice Frederick Sent: Friday, April 21, 2017 8:55 AM To: Elizabeth Jane Lux Subject: RE: sectioning issues? You can always pop by and let me see..... Now would be fine. I am gone all next week. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> From: Elizabeth Jane Lux Sent: Friday, April 21, 2017 8:27 AM To: Bernice Frederick Subject: Re: sectioning issues? Tissue is kidney and heart. Thickness was going off of past work done in the lab, utilizing the guidelines in the AFIP Lab methods in Histo red book. The machine had some maintenance done last year, though the tech seemed really green and unsure of himself. The block seems secure to the cassette. Thanks for your thoughts! Elizabeth Lux Research Tech 3 Feinberg Cardiovascular Research Institute Northwestern University Lurie Building, 10-220 312.503.6368 lab phone 312.503.0219 fax ________________________________ From: Bernice Frederick Sent: Friday, April 21, 2017 7:43 AM To: Elizabeth Jane Lux Subject: RE: sectioning issues? Hi Liz, What kind of tissue are you sectioning? Bear in mind,the microtome you are using may have never had any maintenance on it. And why are you cutting at 6? Unless you are doing neuro or looking for amyloid, 4 um is the best thickness. The block itself may be coming away from the cassette. This can cause said issues. Wiggle the paraffin and see if it is not coming away from the cassette. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu<mailto:b-freder...@northwestern.edu> From: Elizabeth Jane Lux Sent: Thursday, April 20, 2017 4:09 PM To: Bernice Frederick Subject: sectioning issues? Hey Bernice, Wonder if I could ask your opinion on some microtome troubleshooting. I'm having a few tricky paraffin blocks. Facing it gets me nice smooth ribbons, but when I move to the sections (6micron) I get inconsistent results. It will give me a full section, then a half, then full, then half, and so on . . Moved to a test block to work out the kinks, and adjusted the angle slightly, tightened up the blade holder, and things seemed to be ribboning better. But then still having issues with the sample block of interest. Anyway, thought it was worth asking if you had any advice. Thanks! Liz Elizabeth Lux Research Tech 3 Feinberg Cardiovascular Research Institute Northwestern University Lurie Building, 10-220 312.503.6368 lab phone 312.503.0219 fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet