RE: [Histonet] shrinkage during IHC
In my experience, IHC usually plumps tissue back up during reteival. Is this noticed with every antibody or just a few? Certain tissue types? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of zodia...@comcast.net Sent: Saturday, November 06, 2010 8:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] shrinkage during IHC To All, We are a lab that sends our specimens out for IHC and have just switched to another reference laboratory for these services. Our pathologist is saying that the tissue looks shrunk on the IHC slides, yet the slides that I process (HE, and special stains)are fine. Does anyone know what is causing this? The reference lab said it could be the type of slides that I use to mount the sections we send to them. My knowledge in IHC is limited. Also, if this helps, they are FFPE tissue. Thanks for your help Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: SPAM-LOW: [Histonet] shrinkage during IHC
You should have the same morphology from the IHC slides done outside that you get from your HE, do you provide them with slides or the Block? The only thing I can think of is if they airdry after IHC instead of going thru alcohols and xylene to coverslip (if they use AEC or ap/red they may airdry), I have seen cell shrinkage from airdrying after IHC occasionally. Something else just occurred to me, if they over heat during HIER I suppose this could happen, but I have not seen it. Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of zodia...@comcast.net Sent: Saturday, November 06, 2010 6:28 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] shrinkage during IHC To All, We are a lab that sends our specimens out for IHC and have just switched to another reference laboratory for these services. Our pathologist is saying that the tissue looks shrunk on the IHC slides, yet the slides that I process (HE, and special stains)are fine. Does anyone know what is causing this? The reference lab said it could be the type of slides that I use to mount the sections we send to them. My knowledge in IHC is limited. Also, if this helps, they are FFPE tissue. Thanks for your help Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] shrinkage during IHC
To All, We are a lab that sends our specimens out for IHC and have just switched to another reference laboratory for these services. Our pathologist is saying that the tissue looks shrunk on the IHC slides, yet the slides that I process (HE, and special stains)are fine. Does anyone know what is causing this? The reference lab said it could be the type of slides that I use to mount the sections we send to them. My knowledge in IHC is limited. Also, if this helps, they are FFPE tissue. Thanks for your help Jenny ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
