Re: [Histonet] stains for visualizing new bone growth
Robin, I typically stain first with Von Kossa and then counter with MacNeal's. This provides a very nice contrast where obviously mature mineralized bone is black and newly formed bone (osteoid) is grayish-green color. Additionally, your marrow space is nicely contrasted with clear visualization of osteoblasts and osteoclasts lining the bone surface. At the microscope this is a one-stop-shop stain for collecting static bone histomorphometry. Another nice contrasting stain is a modified Goldner's trichrome stain. With this stain cell nuclei are stained first with a Weigert's (iron) hematoxylin, then newly formed bone (osteoid) is stained red with an acid fuchsin/ponceau stain, next an orange G cytoplasmic stain covers the rest and a light green SF yellowish stain follows up with a nice green contrast of the mineralized bone. Very clear differentiation between mineralized bone (green) and newly formed bone (red). This stains works very well with auto threshold functions on some histomorph systems as it has a very nice contrast for the software to recognize. Of course Masson's trichrome works as well but it is typically used on decalcified paraffin embedded sections. I have found the Masson's staining kit at Sigma-Aldrich and kits for all the other stains mentioned can be found at Dorn and Hart Microedge. In fact, Dorn and Hart Microedge (www.dornandhart.com) has a lot to offer now with regards to mineralized bone (hard tissue with or without implant materials) and resin embedded histology. Good luck to you and let me know if you have any additional questions. I would also be happy to share images with you if interested. Jack On Feb 1, 2011, at 1:44 PM, Robin Dean robin_d...@compbio.com wrote: Does anyone know of a good stain to use to clearly show new bone growth other than von Kossa stain? Would appreciate any suggestions anyone might have. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_d...@compbio.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] stains for visualizing new bone growth
Does anyone know of a good stain to use to clearly show new bone growth other than von Kossa stain? Would appreciate any suggestions anyone might have. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_d...@compbio.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] stains for visualizing new bone growth
Robin I'm not sure how accurate this is, but we do a lot of massons trichrome staining in rat muscle pouch studies (they inject into the muscle pouch something that will induce bone formation) and we have noticed that new bone formation has a tendency to stain blue rather than red. You could also try a Goldners trichrome too. That is supposed to stain new bone formation. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robin Dean Sent: Tuesday, February 01, 2011 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] stains for visualizing new bone growth Does anyone know of a good stain to use to clearly show new bone growth other than von Kossa stain? Would appreciate any suggestions anyone might have. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_d...@compbio.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] stains for visualizing new bone growth
You could do a modified tetrachrome stain, this distinguishes newly woven bone. On 2/1/11 2:44 PM, Robin Dean robin_d...@compbio.com wrote: Does anyone know of a good stain to use to clearly show new bone growth other than von Kossa stain? Would appreciate any suggestions anyone might have. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_d...@compbio.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] stains for visualizing new bone growth
You could use May Grunwald-Giemsa modified for tissue (Maximov's procedure). René J. --- On Tue, 2/1/11, Vanessa J. Phelan vjp2...@columbia.edu wrote: From: Vanessa J. Phelan vjp2...@columbia.edu Subject: Re: [Histonet] stains for visualizing new bone growth To: Robin Dean robin_d...@compbio.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, February 1, 2011, 3:54 PM You could do a modified tetrachrome stain, this distinguishes newly woven bone. On 2/1/11 2:44 PM, Robin Dean robin_d...@compbio.com wrote: Does anyone know of a good stain to use to clearly show new bone growth other than von Kossa stain? Would appreciate any suggestions anyone might have. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_d...@compbio.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
