[Histonet] time in paraffin and fried bloody specimen
Good Morning Histonetters- First question: Textbook says tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening. Can anyone define exposure to prolonged heat? Is that an hour? Three hours? Sitting in the paraffin waiting to be drained. I would appreciate some insight on this. Second question: Endom, POC tissue, even some sinus contents arrive wrapped in lens paper. These bloody specimens are fried (for lack of a better word) and almost impossible to separate from the lens paper. Is there something different we or the PA can be doing differently or just the nature of the tissue. Thanks for your help! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] time in paraffin and fried bloody specimen
After you have developed a processing protocol and obtained good infiltration after a certain time (hours) in paraffin, any and all the time above that period of adequate infiltration = exposure to prolonged heat. Some histotechs even don't fill the holding chamber in the embedding center, a practice I do not think is adequate. To your second question, just place them in NBF and when fixed filter and wrap them yourself while cassetting, do not wrap them before being fixed. René J. --- On Mon, 10/5/09, Nancy Schmitt nancy_schm...@pa-ucl.com wrote: From: Nancy Schmitt nancy_schm...@pa-ucl.com Subject: [Histonet] time in paraffin and fried bloody specimen To: Histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Date: Monday, October 5, 2009, 11:06 AM Good Morning Histonetters- First question: Textbook says tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening. Can anyone define exposure to prolonged heat? Is that an hour? Three hours? Sitting in the paraffin waiting to be drained. I would appreciate some insight on this. Second question: Endom, POC tissue, even some sinus contents arrive wrapped in lens paper. These bloody specimens are fried (for lack of a better word) and almost impossible to separate from the lens paper. Is there something different we or the PA can be doing differently or just the nature of the tissue. Thanks for your help! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] time in paraffin and fried bloody specimen
Nancy, Tissue should be processed @ between 60-62 degrees centigrade. We have three paraffin baths. The 1st bath is set for 45 mins, the 2cd and 3rd are for 1 hour each. This is for large specimens. Small specimens are for 30 mins. the first two baths and 45 mins for the last. It is very true that too much time in paraffin causes hard tissue. Remember the whole time the tissue is setting in paraffin it is being exposed to heat. Your second question: Have the specimen transferred from the lens paper it arrived in and put on a new piece which has been moistened with formalin. Sometimes in surgery the lens paper is wet with saline. If it is a scant amount process with your Endoscopic biopsies. Too long in your alcohols will over dehydrate the specimen. Hope this helps. Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical Center I 200 Medical Park Boulevard I Petersburg, Va. 23805 I T: 804-765-5050 I F: 804-765-5582 I dkb...@chs.net Nancy Schmitt nancy_schm...@pa-ucl.com Sent by: histonet-boun...@lists.utsouthwestern.edu 10/05/2009 11:07 AM To Histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu cc Subject [Histonet] time in paraffin and fried bloody specimen Good Morning Histonetters- First question: Textbook says tissue should remain in paraffin the shortest time necessary for good infiltration because exposure to prolonged heat causes shrinkage and hardening. Can anyone define exposure to prolonged heat? Is that an hour? Three hours? Sitting in the paraffin waiting to be drained. I would appreciate some insight on this. Second question: Endom, POC tissue, even some sinus contents arrive wrapped in lens paper. These bloody specimens are fried (for lack of a better word) and almost impossible to separate from the lens paper. Is there something different we or the PA can be doing differently or just the nature of the tissue. Thanks for your help! Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
