AW: [Histonet] C4D Antibody
We work with the Benchmark XT, ultraview kit (polymer), CC1 retrieval (pH 8-9) 30 min, C4d from Zytomed 1:25 32min, with amplification. Gudrun Lang Akh Linz, Austria -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von kk...@bidmc.harvard.edu Gesendet: Freitag, 21. August 2009 19:39 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] C4D Antibody Just wondering if anyone out there routinely works with C4d. I was wondering what you use for retrieval and the Type of detection kit you use. Thanks. Kwadwo Kwaa. BIDMC Pathology Department IHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] C4d Antibody
Hi César we use mo-a-C4d, clone CL314 (Quidel A213) at 1.25 ug/ml (1:800) for cryosections of kidney biopsies (indirect immunofluorescence), it won't work on FFPE material (at least in our hands). For FFPE, Biomedica's rb-a-C4d (BI-RC4D) works fine at ca. 6 ug/ml (1:30) after HIER in a high pH buffer (Tris-EDTA, pH 9.0; Tris-Urea, pH 9.5). Hope this helps. Best regards Andi Kappeler Institute of Pathology, University of Bern, Switzerland -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Cesar Francisco Romero Gesendet: Dienstag, 24. März 2009 05:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] C4d Antibody Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe. I work in a Public Hospital with many kidney transplantations per year. Thank You in advance. César Romero Buenos Aires Argentina _ ¿Querés saber cómo va a estar el clima mañana? Ingresá ahora a MSN http://tiempo.ar.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] C4d Antibody
Our's is from Zytomed, a polyclonal rabbit (a12-5000). We work with the benchmark XT, 1:25 with amplifier. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Cesar Francisco Romero Gesendet: Dienstag, 24. März 2009 05:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] C4d Antibody Dear People from Histonet Could you share your experience with C4d Antibody ? I couldn't get any results with the mouse monoclonal from Quidel. I will try now the rabbit polyclonal ( C4dpAb )from Biomedica - Grouppe. I work in a Public Hospital with many kidney transplantations per year. Thank You in advance. César Romero Buenos Aires Argentina _ ¿Querés saber cómo va a estar el clima mañana? Ingresá ahora a MSN http://tiempo.ar.msn.com/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet