Hi Gudrun
How deep would you consider the oxidation to go? I have tried samples from
around 200um into the block. The Alkaline Alcohol converts the alcian blue
to monastral fast blue, which is insoluble - this is what i have been told.
So locks it in I guess. Doesn't really matter as I don't get alcian blue in
the sample to lock in.
Thanks
Krista
On May 28, 2013 10:09 AM, Gudrun Lang gu.l...@gmx.at wrote:
Hi Krista,
Maybe the bad stainability is more due to oxidation by air of the old
block-surface than due to fixation. A deeper slide for comparison would
show.
I don't know what the alkaline alcohol is good for ?
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Krista
Sider
Gesendet: Montag, 27. Mai 2013 23:28
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Movat Pentachrome - Need to enhance Alcian Blue
Hi All,
I had wonderful help from this site for my Woodstain Scarlet-Acid Fuchsin
binding collagen problem. Now that this is fixed I have realized that the
Alcian Blue is not staining my excessively fixed samples (Human Aorta
Aortic Valve, formalin fixed 2-4 years, embedded in paraffin and stored at
RT for ~6 years). I have some samples fixed for only 2-6 months and these
are fine but the samples fixed for 2-4 years do not uptake the Alcian Blue.
I have taken it up to 1 hr in the 1% Alcian Blue solution followed by 10
min
in 56*C Alkaline Alcohol solution. I do a 1hr Bouin's 50*C to start (I have
also tried Bouin's at 60*C).
Any ideas on how I can get the Alcian Blue to stain these excessively fixed
samples or possibly a substitute? I need to see the proteoglycans/GAGs.
Thank you
Krista
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