RE: [Histonet] Benchmark XT
What kind of slides do you use?? Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, April 09, 2009 6:14 PM To: Kari Bradshaw; histonet@lists.utsouthwestern.edu; Dana Spears; Joe Nocito; Phyllis Thaxton Subject: Re: [Histonet] Benchmark XT I just had the same problem with a bad lot of charged slides. It is, indeed, maddening. Joe Nocito jnoc...@satx.rr.com 4/8/2009 5:08 PM another issue could be the mixer blowing too hard. I've had that happen too. JTT - Original Message - From: Kari Bradshaw kbrads...@lcpath.com To: Dana Spears dspe...@mmci.org; histonet@lists.utsouthwestern.edu; Phyllis Thaxton dch...@yahoo.com Sent: Wednesday, April 08, 2009 2:13 PM Subject: RE: [Histonet] Benchmark XT We just recently went through a similar problem with irregular staining, light staining, sometimes no stain of any kind including counterstain,tissue falling off, and a handfull of false negative patient tissues, but positive controls (on the same slide). With two XT's running continuously it taken several days and everyone's help isolating the problemnot to mention several different lots numbers of slidesyikes! Next time I'll blame the slides right off the bat. Kari L. Bradshaw HT(ASCP) Laboratory Manager Lower Columbia Pathologists (360)425-5620 kbrads...@lcpath.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dana Spears Sent: Wednesday, April 08, 2009 9:24 AM To: histonet@lists.utsouthwestern.edu; Phyllis Thaxton Subject: Re: [Histonet] Benchmark XT I would definitely check your slides - we have found that when we get a bad lot of charged slides, things start washing off on the XT - regardless of detection, antibodies, whatever. It was happening to us on some tissue types and not on others, but a new batch of charged slides did the trick. I've never had to dry 60 min in the oven - 20 does it for us when we aren't having slide issues. Also, if you are using any Sta-on or any adhesive in your waterbath with the charged slides it can cause the slides to sort of repel the tissue and negate the charged effect again certain tissues will fall off, others stay on Dana Spears, HTL(ASCP) Laboratory Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) (309) 279-3768 (fax) dspe...@mmci.org Phyllis Thaxton dch...@yahoo.com 4/8/2009 8:43:36 AM Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies
Re: [Histonet] Benchmark XT
I just had the same problem with a bad lot of charged slides. It is, indeed, maddening. Joe Nocito jnoc...@satx.rr.com 4/8/2009 5:08 PM another issue could be the mixer blowing too hard. I've had that happen too. JTT - Original Message - From: Kari Bradshaw kbrads...@lcpath.com To: Dana Spears dspe...@mmci.org; histonet@lists.utsouthwestern.edu; Phyllis Thaxton dch...@yahoo.com Sent: Wednesday, April 08, 2009 2:13 PM Subject: RE: [Histonet] Benchmark XT We just recently went through a similar problem with irregular staining, light staining, sometimes no stain of any kind including counterstain,tissue falling off, and a handfull of false negative patient tissues, but positive controls (on the same slide). With two XT's running continuously it taken several days and everyone's help isolating the problemnot to mention several different lots numbers of slidesyikes! Next time I'll blame the slides right off the bat. Kari L. Bradshaw HT(ASCP) Laboratory Manager Lower Columbia Pathologists (360)425-5620 kbrads...@lcpath.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dana Spears Sent: Wednesday, April 08, 2009 9:24 AM To: histonet@lists.utsouthwestern.edu; Phyllis Thaxton Subject: Re: [Histonet] Benchmark XT I would definitely check your slides - we have found that when we get a bad lot of charged slides, things start washing off on the XT - regardless of detection, antibodies, whatever. It was happening to us on some tissue types and not on others, but a new batch of charged slides did the trick. I've never had to dry 60 min in the oven - 20 does it for us when we aren't having slide issues. Also, if you are using any Sta-on or any adhesive in your waterbath with the charged slides it can cause the slides to sort of repel the tissue and negate the charged effect again certain tissues will fall off, others stay on Dana Spears, HTL(ASCP) Laboratory Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) (309) 279-3768 (fax) dspe...@mmci.org Phyllis Thaxton dch...@yahoo.com 4/8/2009 8:43:36 AM Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Benchmark XT
I would definitely check your slides - we have found that when we get a bad lot of charged slides, things start washing off on the XT - regardless of detection, antibodies, whatever. It was happening to us on some tissue types and not on others, but a new batch of charged slides did the trick. I've never had to dry 60 min in the oven - 20 does it for us when we aren't having slide issues. Also, if you are using any Sta-on or any adhesive in your waterbath with the charged slides it can cause the slides to sort of repel the tissue and negate the charged effect again certain tissues will fall off, others stay on Dana Spears, HTL(ASCP) Laboratory Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) (309) 279-3768 (fax) dspe...@mmci.org Phyllis Thaxton dch...@yahoo.com 4/8/2009 8:43:36 AM Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Benchmark XT
We just recently went through a similar problem with irregular staining, light staining, sometimes no stain of any kind including counterstain,tissue falling off, and a handfull of false negative patient tissues, but positive controls (on the same slide). With two XT's running continuously it taken several days and everyone's help isolating the problemnot to mention several different lots numbers of slidesyikes! Next time I'll blame the slides right off the bat. Kari L. Bradshaw HT(ASCP) Laboratory Manager Lower Columbia Pathologists (360)425-5620 kbrads...@lcpath.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dana Spears Sent: Wednesday, April 08, 2009 9:24 AM To: histonet@lists.utsouthwestern.edu; Phyllis Thaxton Subject: Re: [Histonet] Benchmark XT I would definitely check your slides - we have found that when we get a bad lot of charged slides, things start washing off on the XT - regardless of detection, antibodies, whatever. It was happening to us on some tissue types and not on others, but a new batch of charged slides did the trick. I've never had to dry 60 min in the oven - 20 does it for us when we aren't having slide issues. Also, if you are using any Sta-on or any adhesive in your waterbath with the charged slides it can cause the slides to sort of repel the tissue and negate the charged effect again certain tissues will fall off, others stay on Dana Spears, HTL(ASCP) Laboratory Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) (309) 279-3768 (fax) dspe...@mmci.org Phyllis Thaxton dch...@yahoo.com 4/8/2009 8:43:36 AM Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
