RE: [Histonet] Biocare decloaker
I swear I blow a gasket at least once a week! Oh wait that's me, not the decloker. :) Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Mon 7/25/2011 3:42 PM To: William; Mike Pence Cc: histonet@lists.utsouthwestern.edu; Diana McCaig Subject: RE: [Histonet] Biocare decloaker I wonder if a new gasket would help me.. I keep blowing mine! :) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William Sent: Monday, July 25, 2011 16:40 To: Mike Pence Cc: histonet@lists.utsouthwestern.edu; Diana McCaig Subject: Re: [Histonet] Biocare decloaker As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:34 PM, Mike Pence mpe...@grhs.net wrote: There is a huge difference between 122 degrees for 30 seconds and 90 degrees for 45 minutes. I would say you need to look at something here! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Monday, July 25, 2011 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare decloaker We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Biocare decloaker
I just sizzle. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, July 27, 2011 3:17 PM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Biocare decloaker I swear I blow a gasket at least once a week! Oh wait that's me, not the decloker. :) Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Mon 7/25/2011 3:42 PM To: William; Mike Pence Cc: histonet@lists.utsouthwestern.edu; Diana McCaig Subject: RE: [Histonet] Biocare decloaker I wonder if a new gasket would help me.. I keep blowing mine! :) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William Sent: Monday, July 25, 2011 16:40 To: Mike Pence Cc: histonet@lists.utsouthwestern.edu; Diana McCaig Subject: Re: [Histonet] Biocare decloaker As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:34 PM, Mike Pence mpe...@grhs.net wrote: There is a huge difference between 122 degrees for 30 seconds and 90 degrees for 45 minutes. I would say you need to look at something here! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Monday, July 25, 2011 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare decloaker We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Biocare decloaker
There is a huge difference between 122 degrees for 30 seconds and 90 degrees for 45 minutes. I would say you need to look at something here! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Monday, July 25, 2011 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare decloaker We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Biocare decloaker
As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:34 PM, Mike Pence mpe...@grhs.net wrote: There is a huge difference between 122 degrees for 30 seconds and 90 degrees for 45 minutes. I would say you need to look at something here! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Monday, July 25, 2011 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare decloaker We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Biocare decloaker
I wonder if a new gasket would help me.. I keep blowing mine! :) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William Sent: Monday, July 25, 2011 16:40 To: Mike Pence Cc: histonet@lists.utsouthwestern.edu; Diana McCaig Subject: Re: [Histonet] Biocare decloaker As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:34 PM, Mike Pence mpe...@grhs.net wrote: There is a huge difference between 122 degrees for 30 seconds and 90 degrees for 45 minutes. I would say you need to look at something here! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Monday, July 25, 2011 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare decloaker We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Biocare decloaker
Ok that is a bigger problem and a very clear answer to your staining issue. Your decloaker should never blow a gasket. Something is wrong and you probably need service or a replacement. Your decloaker is probably getting too hot and over retrieving tissue. I suggest immediately calling Biocare technical support. You need this fixed! Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:42 PM, Weems, Joyce jwe...@sjha.org wrote: I wonder if a new gasket would help me.. I keep blowing mine! :) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William Sent: Monday, July 25, 2011 16:40 To: Mike Pence Cc: histonet@lists.utsouthwestern.edu; Diana McCaig Subject: Re: [Histonet] Biocare decloaker As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:34 PM, Mike Pence mpe...@grhs.net wrote: There is a huge difference between 122 degrees for 30 seconds and 90 degrees for 45 minutes. I would say you need to look at something here! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Monday, July 25, 2011 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare decloaker We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
