Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
Amos: Bryan's method has 2 problems: 1- It requires a volumetric flask (one that assures a constant volume and it does that because the glass cap has a small hole that allows overflow of excess liquid), and 2- it will tell you the density of the liquid you placed inside the flask but NOT its strength, in this case the amount of water the alcohol contains. That would require a table to compare the density you calculated against that of known alcoholic dilutions. For the level of accuracy needed in tissue processing, any hydrometer will suffice, and you don't have to shake it, just wipe it dry with a towel. René J. --- On Fri, 11/26/10, Amos Brooks amosbro...@gmail.com wrote: From: Amos Brooks amosbro...@gmail.com Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS To: Bryan Llewellyn llewl...@shaw.ca Cc: Histonet histonet@lists.utsouthwestern.edu Date: Friday, November 26, 2010, 4:32 PM Wow Bryan, That's pretty slick. Obviously weighing it would do the trick I hadn't thought of that. I'll have to remember that the next time I mindlessly try to shake the excess liquid off my hydrometer and have it break in my hands. Gosh I hated that! That is definitely a great and probably more precise way of doing it. Amos On Thursday 25 November 2010 03:11:07 pm Bryan Llewellyn wrote: Specific gravity is mass/volume. In this context that is grams/millilitre. It can easily be measured without a hydrometer. 1. Obtain a 10 ml beaker and weigh it to 2 decimal places. 2. Measure 10 mL of the alcohol with a volumetric pipette and place in the beaker. 3. Reweigh the beaker with the alcohol in it, again to 2 decimal places. 4. Subtract the weight of the beaker from the weight of the beaker and alcohol, giving the weight of the alcohol 5. Divide the weight of the alcohol by 10 to get the SG to 3 decimal places. Bryan Llewellyn - Original Message - From: Amos Brooks amosbro...@gmail.com To: histonet@lists.utsouthwestern.edu; vickroy@mhsil.com Sent: Thursday, November 25, 2010 3:14 AM Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS Hi Jim, Hydrometers can get really expensive. I searched around for one with a good price and stumbled on this one from Cole Parmer (now Thermo like everyone else in the world): Thermo Scientific ERTCO® Alcohol Hydrometer, 0 to 100% Tralle, 0 to 200 Proof, Plain Form ... CAT# EW-08285-00 I picked it up for $29.50, but that was with my University discount. I'm not sure what regular price is or what discounts you might be able to get. It sure beat the heck out of some of these $200 ones out there. This one has both ETOH percentages as well as ETOH proofs. It works well for us. Happy Thanksgiving, Amos Message: 4 Date: Tue, 23 Nov 2010 13:02:24 -0600 From: Vickroy, Jim vickroy@mhsil.com Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 24a4826e8ef0964d86bc5317306f58a55510fe3...@mmc- mail.ad.mhsil.com Content-Type: text/plain; charset=us-ascii Does anyone know where we can purchase a hydrometer or other instrument for confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in chemicals on a processor and I am going to be asked about instruments to confirm percentages before processing. Meeting with risk management tomorrow. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
Your first objection is not the case. The procedure I outlined used a 10 mL volumetric pipette, not a flask, and a plain beaker. These are extremely common in medical laboratories and accurate enough for the purpose. Your second objection is quite true, but really, how difficult is it to make one time alcohol dilutions and weigh them, then plot the concentrations against either the weights or the specific gravity on a graph. For occasional use it works extremely well. On the other hand, if you are doing it many times a day, then a densitometer with direct readings of acohol concentrations should be used. Bryan Llewellyn - Original Message - From: Rene J Buesa To: Bryan Llewellyn ; Amos Brooks Cc: Histonet Sent: Sunday, November 28, 2010 6:20 AM Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS Amos: Bryan's method has 2 problems: 1- It requires a volumetric flask (one that assures a constant volume and it does that because the glass cap has a small hole that allows overflow of excess liquid), and 2- it will tell you the density of the liquid you placed inside the flask but NOT its strength, in this case the amount of water the alcohol contains. That would require a table to compare the density you calculated against that of known alcoholic dilutions. For the level of accuracy needed in tissue processing, any hydrometer will suffice, and you don't have to shake it, just wipe it dry with a towel. René J. --- On Fri, 11/26/10, Amos Brooks amosbro...@gmail.com wrote: From: Amos Brooks amosbro...@gmail.com Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS To: Bryan Llewellyn llewl...@shaw.ca Cc: Histonet histonet@lists.utsouthwestern.edu Date: Friday, November 26, 2010, 4:32 PM Wow Bryan, That's pretty slick. Obviously weighing it would do the trick I hadn't thought of that. I'll have to remember that the next time I mindlessly try to shake the excess liquid off my hydrometer and have it break in my hands. Gosh I hated that! That is definitely a great and probably more precise way of doing it. Amos On Thursday 25 November 2010 03:11:07 pm Bryan Llewellyn wrote: Specific gravity is mass/volume. In this context that is grams/millilitre. It can easily be measured without a hydrometer. 1. Obtain a 10 ml beaker and weigh it to 2 decimal places. 2. Measure 10 mL of the alcohol with a volumetric pipette and place in the beaker. 3. Reweigh the beaker with the alcohol in it, again to 2 decimal places. 4. Subtract the weight of the beaker from the weight of the beaker and alcohol, giving the weight of the alcohol 5. Divide the weight of the alcohol by 10 to get the SG to 3 decimal places. Bryan Llewellyn - Original Message - From: Amos Brooks amosbro...@gmail.com To: histonet@lists.utsouthwestern.edu; vickroy@mhsil.com Sent: Thursday, November 25, 2010 3:14 AM Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS Hi Jim, Hydrometers can get really expensive. I searched around for one with a good price and stumbled on this one from Cole Parmer (now Thermo like everyone else in the world): Thermo Scientific ERTCO® Alcohol Hydrometer, 0 to 100% Tralle, 0 to 200 Proof, Plain Form ... CAT# EW-08285-00 I picked it up for $29.50, but that was with my University discount. I'm not sure what regular price is or what discounts you might be able to get. It sure beat the heck out of some of these $200 ones out there. This one has both ETOH percentages as well as ETOH proofs. It works well for us. Happy Thanksgiving, Amos Message: 4 Date: Tue, 23 Nov 2010 13:02:24 -0600 From: Vickroy, Jim vickroy@mhsil.com Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 24a4826e8ef0964d86bc5317306f58a55510fe3...@mmc- mail.ad.mhsil.com Content-Type: text/plain; charset=us-ascii Does anyone know where we can purchase a hydrometer or other instrument for confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in chemicals on a processor and I am going to be asked about instruments to confirm percentages before processing
Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
This is what I meant: http://en.wikipedia.org/wiki/Volumetric_pipettes Bryan Llewellyn - Original Message - From: Lynn Lee To: llewl...@shaw.ca Sent: Sunday, November 28, 2010 7:53 AM Subject: RE: [Histonet] CHECKING ALCOHOL CONCENTRATIONS Do you mean a calibrated pipette? L. Lee, Tucson, AZ From: llewl...@shaw.ca To: histonet@lists.utsouthwestern.edu; rjbu...@yahoo.com Date: Sun, 28 Nov 2010 07:46:52 -0800 Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS CC: Your first objection is not the case. The procedure I outlined used a 10 mL volumetric pipette, not a flask, and a plain beaker. These are extremely common in medical laboratories and accurate enough for the purpose. Your second objection is quite true, but really, how difficult is it to make one time alcohol dilutions and weigh them, then plot the concentrations against either the weights or the specific gravity on a graph. For occasional use it works extremely well. On the other hand, if you are doing it many times a day, then a densitometer with direct readings of acohol concentrations should be used. Bryan Llewellyn - Original Message - From: Rene J Buesa To: Bryan Llewellyn ; Amos Brooks Cc: Histonet Sent: Sunday, November 28, 2010 6:20 AM Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS Amos: Bryan's method has 2 problems: 1- It requires a volumetric flask (one that assures a constant volume and it does that because the glass cap has a small hole that allows overflow of excess liquid), and 2- it will tell you the density of the liquid you placed inside the flask but NOT its strength, in this case the amount of water the alcohol contains. That would require a table to compare the density you calculated against that of known alcoholic dilutions. For the level of accuracy needed in tissue processing, any hydrometer will suffice, and you don't have to shake it, just wipe it dry with a towel. René J. --- On Fri, 11/26/10, Amos Brooks amosbro...@gmail.com wrote: From: Amos Brooks amosbro...@gmail.com Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS To: Bryan Llewellyn llewl...@shaw.ca Cc: Histonet histonet@lists.utsouthwestern.edu Date: Friday, November 26, 2010, 4:32 PM Wow Bryan, That's pretty slick. Obviously weighing it would do the trick I hadn't thought of that. I'll have to remember that the next time I mindlessly try to shake the excess liquid off my hydrometer and have it break in my hands. Gosh I hated that! That is definitely a great and probably more precise way of doing it. Amos On Thursday 25 November 2010 03:11:07 pm Bryan Llewellyn wrote: Specific gravity is mass/volume. In this context that is grams/millilitre. It can easily be measured without a hydrometer. 1. Obtain a 10 ml beaker and weigh it to 2 decimal places. 2. Measure 10 mL of the alcohol with a volumetric pipette and place in the beaker. 3. Reweigh the beaker with the alcohol in it, again to 2 decimal places. 4. Subtract the weight of the beaker from the weight of the beaker and alcohol, giving the weight of the alcohol 5. Divide the weight of the alcohol by 10 to get the SG to 3 decimal places. Bryan Llewellyn - Original Message - From: Amos Brooks amosbro...@gmail.com To: histonet@lists.utsouthwestern.edu; vickroy@mhsil.com Sent: Thursday, November 25, 2010 3:14 AM Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS Hi Jim, Hydrometers can get really expensive. I searched around for one with a good price and stumbled on this one from Cole Parmer (now Thermo like everyone else in the world): Thermo Scientific ERTCO® Alcohol Hydrometer, 0 to 100% Tralle, 0 to 200 Proof, Plain Form ... CAT# EW-08285-00 I picked it up for $29.50, but that was with my University discount. I'm not sure what regular price is or what discounts you might be able to get. It sure beat the heck out of some of these $200 ones out there. This one has both ETOH percentages as well as ETOH proofs. It works well for us. Happy Thanksgiving, Amos Message: 4 Date: Tue, 23 Nov 2010 13:02:24 -0600 From: Vickroy, Jim vickroy@mhsil.com Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 24a4826e8ef0964d86bc5317306f58a55510fe3...@mmc- mail.ad.mhsil.com Content-Type: text/plain; charset=us-ascii Does anyone know where we can purchase a hydrometer or other instrument for confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in chemicals on a processor and I am going to be asked about instruments to confirm percentages before
Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
Wow Bryan, That's pretty slick. Obviously weighing it would do the trick I hadn't thought of that. I'll have to remember that the next time I mindlessly try to shake the excess liquid off my hydrometer and have it break in my hands. Gosh I hated that! That is definitely a great and probably more precise way of doing it. Amos On Thursday 25 November 2010 03:11:07 pm Bryan Llewellyn wrote: Specific gravity is mass/volume. In this context that is grams/millilitre. It can easily be measured without a hydrometer. 1. Obtain a 10 ml beaker and weigh it to 2 decimal places. 2. Measure 10 mL of the alcohol with a volumetric pipette and place in the beaker. 3. Reweigh the beaker with the alcohol in it, again to 2 decimal places. 4. Subtract the weight of the beaker from the weight of the beaker and alcohol, giving the weight of the alcohol 5. Divide the weight of the alcohol by 10 to get the SG to 3 decimal places. Bryan Llewellyn - Original Message - From: Amos Brooks amosbro...@gmail.com To: histonet@lists.utsouthwestern.edu; vickroy@mhsil.com Sent: Thursday, November 25, 2010 3:14 AM Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS Hi Jim, Hydrometers can get really expensive. I searched around for one with a good price and stumbled on this one from Cole Parmer (now Thermo like everyone else in the world): Thermo Scientific ERTCO® Alcohol Hydrometer, 0 to 100% Tralle, 0 to 200 Proof, Plain Form ... CAT# EW-08285-00 I picked it up for $29.50, but that was with my University discount. I'm not sure what regular price is or what discounts you might be able to get. It sure beat the heck out of some of these $200 ones out there. This one has both ETOH percentages as well as ETOH proofs. It works well for us. Happy Thanksgiving, Amos Message: 4 Date: Tue, 23 Nov 2010 13:02:24 -0600 From: Vickroy, Jim vickroy@mhsil.com Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 24a4826e8ef0964d86bc5317306f58a55510fe3...@mmc- mail.ad.mhsil.com Content-Type: text/plain; charset=us-ascii Does anyone know where we can purchase a hydrometer or other instrument for confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in chemicals on a processor and I am going to be asked about instruments to confirm percentages before processing. Meeting with risk management tomorrow. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
Specific gravity is mass/volume. In this context that is grams/millilitre. It can easily be measured without a hydrometer. 1. Obtain a 10 ml beaker and weigh it to 2 decimal places. 2. Measure 10 mL of the alcohol with a volumetric pipette and place in the beaker. 3. Reweigh the beaker with the alcohol in it, again to 2 decimal places. 4. Subtract the weight of the beaker from the weight of the beaker and alcohol, giving the weight of the alcohol 5. Divide the weight of the alcohol by 10 to get the SG to 3 decimal places. Bryan Llewellyn - Original Message - From: Amos Brooks amosbro...@gmail.com To: histonet@lists.utsouthwestern.edu; vickroy@mhsil.com Sent: Thursday, November 25, 2010 3:14 AM Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS Hi Jim, Hydrometers can get really expensive. I searched around for one with a good price and stumbled on this one from Cole Parmer (now Thermo like everyone else in the world): Thermo Scientific ERTCO® Alcohol Hydrometer, 0 to 100% Tralle, 0 to 200 Proof, Plain Form ... CAT# EW-08285-00 I picked it up for $29.50, but that was with my University discount. I'm not sure what regular price is or what discounts you might be able to get. It sure beat the heck out of some of these $200 ones out there. This one has both ETOH percentages as well as ETOH proofs. It works well for us. Happy Thanksgiving, Amos Message: 4 Date: Tue, 23 Nov 2010 13:02:24 -0600 From: Vickroy, Jim vickroy@mhsil.com Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 24a4826e8ef0964d86bc5317306f58a55510fe3...@mmc- mail.ad.mhsil.com Content-Type: text/plain; charset=us-ascii Does anyone know where we can purchase a hydrometer or other instrument for confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in chemicals on a processor and I am going to be asked about instruments to confirm percentages before processing. Meeting with risk management tomorrow. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
Fisher Scientific. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Vickroy, Jim vickroy@mhsil.com Sent by: histonet-boun...@lists.utsouthwestern.edu 11/23/2010 02:07 PM To histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] CHECKING ALCOHOL CONCENTRATIONS Does anyone know where we can purchase a hydrometer or other instrument for confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in chemicals on a processor and I am going to be asked about instruments to confirm percentages before processing. Meeting with risk management tomorrow. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
CBG Biotech. Their contact info is 1-800-941-9484 or you can e mail them at i...@cbgbiotech.com. I purchased my recycler through them and have ordered extra hydrometers from them, too. They give excellent customer service. Sandy Harrison, HTL(ASCP) Histology Supervisor, VA Minneapolis 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dkb...@chs.net Sent: Tuesday, November 23, 2010 2:48 PM To: Vickroy, Jim Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS Fisher Scientific. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Vickroy, Jim vickroy@mhsil.com Sent by: histonet-boun...@lists.utsouthwestern.edu 11/23/2010 02:07 PM To histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] CHECKING ALCOHOL CONCENTRATIONS Does anyone know where we can purchase a hydrometer or other instrument for confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in chemicals on a processor and I am going to be asked about instruments to confirm percentages before processing. Meeting with risk management tomorrow. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CHECKING ALCOHOL CONCENTRATIONS
Lab Safety Supply www.lss.com Cole-Parmer www.coleparmer.com Fisher Scientific www.fishersci.com Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -- From: Vickroy, Jim vickroy@mhsil.com Sent: Tuesday, November 23, 2010 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CHECKING ALCOHOL CONCENTRATIONS Does anyone know where we can purchase a hydrometer or other instrument for confirming alcohol percentages, such as 70, 85, 95, 100? We had a mixup in chemicals on a processor and I am going to be asked about instruments to confirm percentages before processing. Meeting with risk management tomorrow. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
