RE: [Histonet] FNA SLIDES
Adequacy is carried out by the Biomedical Scientist usually in the UK. I make a couple of air dried per passage and stain Diff Quick; you can then give an indication of adequacy within 5 min or so. Usually if the FNA is performed properly you don't get much material;; for example breast and LN's are usually sparse and you get maybe a couple of slides (we don't check these for adequacy as the Site is easily re-needled). Pancreas, Thyroid and Lung are another matter. Usually CT orientated FNAC of the lung tend not to be too cellular but sometimes they are; I used to stain a couple for adequacy and make as many slides as possible. The problem is if you make loads of slides and malignant cells are not apparent in those you stain then you have to look at all the others; tough. If there are malignant cells abundant then you ought to look at all the slides just in case they hold diagnostic information. In the end we did 6 slides and washed the remainder into a pot with saline and did Cytospins if appropriate. Pancreatic FNACs taken under a ultra sound flexible scope can also be very cellular (blood) and the above statement holds. Thyroid's can be very bloody, very bloody; trick is not to pull on the plunger but let it seep into the syringe. Too much blood and what little you have gets well diluted!! Kemlo Rogerson e-mail kemloroger...@nhs.net if not at work. DD 01934 647057 or extension 3311 Mob 07749 754194; Embrace uncertainty. Hard problems rarely have easy solutions. --Jonah Lehrer This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jane C. Moose Sent: 31 August 2009 19:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FNA SLIDES A question has arisen for us- How many slides do you (should you) make per pass for pathologist for adequacy and/or diagnosis? What about CT guided biopsies of liver, lung, masses etc. Thanks in advance for your input. Jane Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FNA SLIDES
A lot depends on the pathologist and the nature of the specimen. For CT guided biopsies where you get a core and do touch preps. One slide per pass should be sufficient. For FNA's where you get fluid I make two slides per staining medium (some pathologists want Toluene Blue and Diff Quick. Some want a Diff Quik and a Pap Stain). The remainder of the fluid should be put into CytoLyt or other suitable fluid for cell block and/or Liquid Based Cytology processing. It is possible to do a rapid Pap that while not nearly as quick as the Diff Quick is still doable. The bottom line is that it is up to the pathologist that has to look at them. Hope this helps. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jane C. Moose Sent: Monday, August 31, 2009 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FNA SLIDES A question has arisen for us- How many slides do you (should you) make per pass for pathologist for adequacy and/or diagnosis? What about CT guided biopsies of liver, lung, masses etc. Thanks in advance for your input. Jane Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FNA SLIDES
We make two slides. One air dried DQ for immediate assessment, second alcohol fixed for PAP in the lab. The spreader we either stain with Giemsa or rehydrate (using saline) and stain with PAP in lab. Triage as follows: If inflammatory, second pass for microbiology. If malignant, if required, then second pass for cell block. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jane C. Moose Sent: Tuesday, 1 September 2009 4:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FNA SLIDES A question has arisen for us- How many slides do you (should you) make per pass for pathologist for adequacy and/or diagnosis? What about CT guided biopsies of liver, lung, masses etc. Thanks in advance for your input. Jane Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
