RE: [Histonet] Formula 83: xylene substitute
Kiran, We use Formula 83 on both staining and processing without any problem at all. You have to watch and either rotate or change your last Formula 83s on the stain line because you can't tell if there is water in it like you can with xylene. We also use CBG's recycler to recycle Formula 83 without any problem. We have not had any problems with our IHC using Formula 83 either. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kiran Sent: Tuesday, May 14, 2013 9:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formula 83: xylene substitute Hello Everyone, I would like to get some feedback from Formula 83 users. Are you using it only on the stainers or for tissue processing as well. What kind of validation process is required to switch to this substitute for ihc stains? We are interested to use it for both histo and cyto lab. Any input is much appreciated. Thank you in advance, Kiran Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formula 83: xylene substitute
Thanks, Linda. What about the odor? Does it have strong or irritating odor? How did you staff responded to the change? We would like to know all before trying it out. Currenly, our staff is having issues with Xylene fumes and goal is to limit any fumes or exposure to Xylene. I would like to hear from others who tried to use it and didn't work for them. -Kiran --- On Tue, 5/14/13, Blazek, Linda lbla...@digestivespecialists.com wrote: From: Blazek, Linda lbla...@digestivespecialists.com Subject: RE: [Histonet] Formula 83: xylene substitute To: Kiran kira...@sbcglobal.net, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, May 14, 2013, 7:13 AM Kiran, We use Formula 83 on both staining and processing without any problem at all. You have to watch and either rotate or change your last Formula 83s on the stain line because you can't tell if there is water in it like you can with xylene. We also use CBG's recycler to recycle Formula 83 without any problem. We have not had any problems with our IHC using Formula 83 either. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kiran Sent: Tuesday, May 14, 2013 9:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formula 83: xylene substitute Hello Everyone, I would like to get some feedback from Formula 83 users. Are you using it only on the stainers or for tissue processing as well. What kind of validation process is required to switch to this substitute for ihc stains? We are interested to use it for both histo and cyto lab. Any input is much appreciated. Thank you in advance, Kiran Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formula 83
Phyllis, I have used Formula 83 for several years and have no problem with it. I do use it just like xylene. As for how frequently you need to change it on the processor really depends on your volume of processing. I didn't have to change the frequency when I switched from xylene to Formula 83. I also recycle my Formula 83. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Phyllis Thaxton Sent: Wednesday, July 22, 2009 10:04 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formula 83 Does anyone have any experience with Formula 83. We are evaluation it now and I would like to know how frequently it needs to be changed on the processor. The label says use just like xylene...BUT is there anyone out in HistoLand that uses it? I would like to know your experience in processing. Right now we are using it on a VIP. Thanks!!! Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formula 83 users?
Jacqueline: Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant. René J. --- On Wed, 4/8/09, Jacqueline Farnsworth jacqueline.farnswo...@cls.ab.ca wrote: From: Jacqueline Farnsworth jacqueline.farnswo...@cls.ab.ca Subject: [Histonet] Formula 83 users? To: Histonet Histonet@lists.utsouthwestern.edu Date: Wednesday, April 8, 2009, 12:31 PM HI all. I have searched the Histonet archives and found a lot of very positive reviews regarding Formula 83. I am wondering if anyone has encountered any issues with using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there any miscibility issues? Are you soaking in xylene prior to tape coverslipping required? Any helpful hints/tips/tricks? Thank you in advance. Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formula 83 users?
With all due respect René; I have been using Formula 83 with my coverslipper with no problems at all though have a glass coverslipper not a tape. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lbla...@digestivespecialists.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, April 09, 2009 9:56 AM To: Histonet; Jacqueline Farnsworth Subject: Re: [Histonet] Formula 83 users? Jacqueline: Formula 83 is a naphthenic hydrocarbon that cannot be used with coverslippers, is recyclable and while some people have been using it for more than 20 years, others find it irritant. René J. --- On Wed, 4/8/09, Jacqueline Farnsworth jacqueline.farnswo...@cls.ab.ca wrote: From: Jacqueline Farnsworth jacqueline.farnswo...@cls.ab.ca Subject: [Histonet] Formula 83 users? To: Histonet Histonet@lists.utsouthwestern.edu Date: Wednesday, April 8, 2009, 12:31 PM HI all. I have searched the Histonet archives and found a lot of very positive reviews regarding Formula 83. I am wondering if anyone has encountered any issues with using Formula 83 on a stainer and then xylene on a tape coverslipper. Are there any miscibility issues? Are you soaking in xylene prior to tape coverslipping required? Any helpful hints/tips/tricks? Thank you in advance. Jacqueline Farnsworth Anatomic Pathology, Tech III Foothills Medical Centre Calgary Laboratory Services Ph: 403-944-1578 Fax: 403-944-4748 P Please consider the environment before printing this email. This message and any attached documents are only for the use of the intended recipient(s), are confidential and may contain privileged information. Any unauthorized review, use, retransmission, or other disclosure is strictly prohibited. If you have received this message in error, please notify the sender immediately, and then delete the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
