Re: [Histonet] her 2 controls

[Dessoye, Michael via Histonet]

Check out Histocyte controls, which are available on slides or blocks.  One of 
their distributors is Cell Marque:

http://www.cellmarque.com/cms/histocyte/HER2.php

Their Her2 control include 0, 1+, 2+, and 3+.

Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | 
Commonwealth Health Laboratory Services | mjdess...@commonwealthhealth.net | 
575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 
570-552-1484

-Original Message-
From: Nirmala Srishan [mailto:sris...@mail.holyname.org] 
Sent: Thursday, April 18, 2019 12:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] her 2 controls

Can someone suggest a company to purchase good Her 2 control slides. 
Preferably multi-tissue controls with +1,+2,+3.
Thanks in advance.


Nirmala Srishan
Supervisor Histology
Department of Pathology and Laboratory Medicine Holy Name Medical Center 
Teaneck, NJ
Office: 201 541 6328
Lab: 201 833 3023
sris...@holyname.org







Holy Name Medical Center is ranked among the top hospitals in the nation 
for patient care, clinical performance and workplace excellence.
Click here to learn more.

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Re: [Histonet] her 2 controls

[John Garratt via Histonet]

I recommend that you e-mail these two gentlemen (both are super helpful) and 
they can put you in touch with your local distributor. Both their companies 
have HER2 controls plus controls for many other analytes.

mr...@statlab.com   (Mark Rees)

colin.trist...@histocyte.com


John


www.ciqc.ca

‐‐‐ Original Message ‐‐‐
On Thursday, April 18, 2019 9:15 AM, Nirmala Srishan via Histonet 
 wrote:

> Can someone suggest a company to purchase good Her 2 control slides.
> Preferably multi-tissue controls with +1,+2,+3.
> Thanks in advance.
>
> Nirmala Srishan
> Supervisor Histology
> Department of Pathology and Laboratory Medicine
> Holy Name Medical Center Teaneck, NJ
> Office: 201 541 6328
> Lab: 201 833 3023
> sris...@holyname.org
>
> Holy Name Medical Center is ranked among the top hospitals in the nation
> for patient care, clinical performance and workplace excellence.
> Click here to learn more.
>
>  Warning: The information contained in this message is privileged and
> CONFIDENTIAL and is intended only for the use of the addressee above. If
> you are not the intended recipient, you are hereby notified that any
> disclosure, copying, distribution, or taking of any action in reliance on
> the content of this message is strictly prohibited. If you have received
> this communication in error, please notify the sender by replying to this
> message, and then delete it from your system.
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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Re: [Histonet] Her 2 Control

[Terri Braud via Histonet]

Hey Scott - Our interpretation of CAP requirements for IHC Controls is the same 
as yours.  Our Her2 control is a microarray with  3+ case and a piece of normal 
skin to serve as our negative tissue control.
If you have normal breast tissue adjacent to your tumor control, that can also 
serve as a negative tissue control, but your procedure has to state it as such. 
 Our control tissue are 5mm punches of solid tumor, so we just add a punch of 
normal skin. 
I hope this helps, but sometimes, it's just not worth the argument.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal


Message: 2
Date: Tue, 21 Nov 2017 21:05:51 +
From: "Lindrud, Scott" 
Subject: [Histonet] Negative IHC Control for Her2
Hi All,
We are having an internal debate regarding Her2 IHC control tissue in our lab.  
We run a MTA (multi tissue array) consisting of 0+, 1+, 2+, and 3+ Her2 
staining tissue taken from lumpectomy/resection cases.  I'm in the process of 
searching for more tissue to use in future control blocks and it can be 
difficult to find tissue that is 0+ and 3+.
I've discussed this with our pathologist in charge of Histology and he says 
that we don't need to run all 4 of the cores as controls.  He says all we need 
to run is a positive control and a negative control.  He says the positive 
control could be a 2+ or 3+ and the negative control could be either a 0+ OR a 
1+.
I respectfully disagreed with him and said the negative control is not meant 
for accessing staining interpretation but to verify the sensitivity and 
specificity of the actual antibody-antigen reaction.  I said a 1+ is still 
staining the tissue where there is no staining in a 0+ reaction.  The 1+ is not 
a negative staining reaction, but a negative interpretation.   The pathologist 
says I'm wrong.
The CAP in its checklist says "It is also important to assess the specificity 
of each antibody by a negative tissue control, which must show no staining of 
tissues known to lack the antigen".To me, a 1+ Her2 staining reaction shows 
that that tissue has antigen and should not be used as a negative control.
So, after saying all that, can/should  1+ Her2 breast tissue be used as a 
negative tissue control?  Seems pretty straight forward to me, but I'm just a 
Cytotechnologist/Histotech.
Thanks for any input!
Scott
Scott A. Lindrud, MLS(ASCP)CM CTCM
Histopathology Technical Specialist/Cytotechnologist Rice Memorial Hospital
301 Becker Ave SW
Willmar, MN 56201
WP(320)231-4406
Fax(320)231-4861
scott.lind...@rice.willmar.mn.us




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Message: 3
Date: Wed, 22 Nov 2017 16:02:21 + (UTC)
From: Rene J Buesa 
To: "Lindrud, Scott" ,
"histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] Negative IHC Control for Her2
Message-ID: <800857894.1231622.1511366541...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

You are right but, your point is?Regardless, the pathologist is the responsible 
for his/her diagnosis/interpretation and the liability is his/hers.Remember 
that for us histotechs, our "client" is the pathologist (always remembering the 
patient behind the whole process) and our essential duty is to provide the 
pathologist what s/he needs to being able to make the best and more accurate 
diagnosis.Follow his/her instructions and your life will be much simpler and 
less stressful.Ren?

On Tuesday, November 21, 2017 4:21 PM, "Lindrud, Scott via Histonet" 
 wrote:


 Hi All,

We are having an internal debate regarding Her2 IHC control tissue in our lab.? 
We run a MTA (multi tissue array) consisting of 0+, 1+, 2+, and 3+ Her2 
staining tissue taken from lumpectomy/resection cases.? I'm in the process of 
searching for more tissue to use in future control blocks and it can be 
difficult to find tissue that is 0+ and 3+.

I've discussed this with our pathologist in charge of Histology and he says 
that we don't need to run all 4 of the cores as controls.? He says all we need 
to run is a positive control and a negative control.? He says the positive 
control could be a 2+ or 3+ and the negative control could be either a 0+ OR a 
1+.

I respectfully disagreed with him and said the negative control is not meant 
for accessing staining interpretation but to verify the sensitivity and 
specificity of 

Re: [Histonet] HER-2

[Rene J Buesa]

Yes, that is sometimes a common occurrence amongst pathologists, BUT those 
differences have to be solved in conference before issuing the report.
Difficult cases (at least at my hospital) are reviewed in conference,
I agree with you: your protocol (specially if it is based on DAKO's protocol) 
should remain as is.
The diagnosis differences should not determine a change.
René J.

From: Mark Tarango marktara...@gmail.com
To: Wilson A wilson6...@yahoo.com 
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Tuesday, February 5, 2013 2:24 PM
Subject: Re: [Histonet] HER-2

I'd be interested to know if all your pathologists agree that the 2+ cases
are 2+.  Is it possible that one of the pathologists is calling more cases
as 2+ than the rest?  I would have a lot of questions before modifying the
staining protocol.  It would be helpful you posted more info.

Mark

On Fri, Feb 1, 2013 at 6:52 PM, Wilson A wilson6...@yahoo.com wrote:


    Hi,
      Our pathologists are concerned we may be reporting too many 2+
 HER2’s.  Can someone  help with this?

      Thanks,
    Wilson
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Re: [Histonet] HER-2

[Mark Tarango]

Many pathologists, if they have any doubt about the score will just say
that it is 2+ so that its gets HER2 by FISH which is considered the best
method for determining HER2 status.  Even if by the scoring criteria it is
a 1+ but the intensity is a little stronger than normal (but maybe
basolateral or not complete staining) they just go with 2+.  Sometimes its
high grade 1+ and it doesn't quite meet the 2+ staining criteria and they
call it 2+ too.  If these things are happening enough it could mean calling
more 2+ cases.  They don't always follow the scoring criteria to the letter.

We use digital image analysis for HER2 IHC scoring and the computer is
pretty right on (matches with FISH), but sometimes the pathologist will
change the score in the report to 2+ even though it's a 1+ by the computer.
 There is one pathologist in particular who doesn't believe in the computer
reading the HER2 score and is trying very hard to find cases that are
positive by FISH but that the computer is calling the IHC 1+.  He also
requests FISH on some 3+ cases to try and find any over-calling by the
computer.  The thought of a woman getting a toxic drug needlessly really
bothers him.

So without Wilson posting more info there's not much help that I think
anyone can offer.  This is stain that has to be validated
more extensively than others, so the protocol shouldn't just be tweaked.
 How do the controls look?  Was there any lot to lot variation?  Lots of
questions..

Mark

On Tue, Feb 5, 2013 at 12:49 PM, Rene J Buesa rjbu...@yahoo.com wrote:

 Yes, that is sometimes a common occurrence amongst pathologists, BUT those
 differences have to be solved in conference before issuing the report.
 Difficult cases (at least at my hospital) are reviewed in conference,
 I agree with you: your protocol (specially if it is based on DAKO'sprotocol) 
 should remain as is.
 The diagnosis differences should not determine a change.
 René J.

   *From:* Mark Tarango marktara...@gmail.com
 *To:* Wilson A wilson6...@yahoo.com
 *Cc:* histonet@lists.utsouthwestern.edu 
 histonet@lists.utsouthwestern.edu
 *Sent:* Tuesday, February 5, 2013 2:24 PM
 *Subject:* Re: [Histonet] HER-2

 I'd be interested to know if all your pathologists agree that the 2+ cases
 are 2+.  Is it possible that one of the pathologists is calling more cases
 as 2+ than the rest?  I would have a lot of questions before modifying the
 staining protocol.  It would be helpful you posted more info.

 Mark T.

 On Fri, Feb 1, 2013 at 6:52 PM, Wilson A wilson6...@yahoo.com wrote:

 
 Hi,
   Our pathologists are concerned we may be reporting too many 2+
  HER2’s.  Can someone  help with this?
 
   Thanks,
 Wilson
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RE: [Histonet] HER-2

[Weems, Joyce K.]

I would suggest that you send several of your own cases to a reference lab that 
reads tons of them and see what numbers they report. Then compare the two 
results.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



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Atlanta, GA 30342

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wilson A
Sent: Friday, February 01, 2013 9:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HER-2


Hi,
  Our pathologists are concerned we may be reporting too many 2+ HER2’s.  
Can someone  help with this?

 Thanks,
Wilson
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Re: [Histonet] Her-2 procedure

[Rene J Buesa]

The protocol described by DAKO is very explicit, it produces consistent results 
and is the one I followed.
Check it out.
René J.

From: Wilson A wilson6...@yahoo.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Sunday, February 3, 2013 12:20 AM
Subject: [Histonet] Her-2 procedure

 
 
 Hi,
 Please I will appreciate it, if you guys could share your HER-2  PROCEDURE 
with me.
 
Thanks,
Wilson
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Re: [Histonet] HER-2

[Rene J Buesa]

That is a very strange question by your pathologists, since they are the ones 
doing the reporting.
If they follow the reporting guidelines and arrive to a 2+ result, they should 
report it.
Another question would be: are our lab's 2+ reports above the mean for other 
labs? The answer to this question can be done only if your find out the 2+ 
percentage for all labs is, and compare with yours.
I think that your pathologists should contact CAP to find out if there are 
statistics on this issue.
René J.

From: Wilson A wilson6...@yahoo.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Friday, February 1, 2013 9:52 PM
Subject: [Histonet] HER-2

 
    Hi,
  Our pathologists are concerned we may be reporting too many 2+ HER2’s.  
Can someone  help with this?
 
 Thanks,
    Wilson
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RE: [Histonet] Her/2

[anita dudley]

marcia,  was just getting ready to stain our her 2's ,  will let you know how 
they look.
 
anita dudley
 providence hosp
mobile,  al
 Date: Wed, 4 May 2011 12:54:29 -0400
 From: fu...@mercyhealth.com
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Her/2
 
 CAP validation Her/2 slides not staining as clear as before. Is anyone else 
 having any issues ? We use the Ventana system and our daily IHC slides are 
 staining
 and controls are working well. Is there a coating on these slides from CAP ? 
 Thanks for any help ?
 Marcia Funk HT,QIHC
 
 
 
 Marcia Funk 
 Histology Laboratory
 Mercy Medical Center North Iowa
 Mason City, IA, 50401
 641-428-7907
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Re: [Histonet] Her/2

[Mark Tarango]

Hi Marcia,

Our slides for the Her2 CAP survey stained just fine.  We're using a Ventana
XT to stain for Her2 by IHC.

Mark
On Wed, May 4, 2011 at 9:54 AM, Marcia Funk fu...@mercyhealth.com wrote:

 CAP validation Her/2 slides not staining as clear as before.  Is anyone
 else having any issues ? We use the Ventana system and our daily IHC slides
 are staining
 and controls are working well.  Is there a coating on these slides from CAP
 ?
 Thanks for any help ?
 Marcia Funk HT,QIHC



 Marcia Funk
 Histology Laboratory
 Mercy Medical Center North Iowa
 Mason City, IA, 50401
 641-428-7907
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Re: [Histonet] Her/2

[Richard Cartun]

Yes, our slides look weak.  I did slides for another hospital and they look 
weak as well.  I have already contacted the CAP to see if they have received 
any other complaints.

Richard

Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


 Marcia Funk fu...@mercyhealth.com 5/4/2011 12:54 PM 
CAP validation Her/2 slides not staining as clear as before.  Is anyone else 
having any issues ? We use the Ventana system and our daily IHC slides are 
staining
and controls are working well.  Is there a coating on these slides from CAP ? 
Thanks for any help ?
Marcia Funk HT,QIHC
 
 
 
Marcia Funk 
Histology Laboratory
Mercy Medical Center North Iowa
Mason City, IA, 50401
641-428-7907
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RE: [Histonet] HER 2

[Fawn Bomar]

I-VIEW DAB Detection Kit from Ventena


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org 
[sbald...@mhhcc.org]
Sent: Wednesday, September 22, 2010 3:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HER 2

Which detection kit do you use?

Thanks
Pathology Supervisor
Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbald...@mhhcc.org
Ph 812-482-0210, 482-0216,  Fax 812-482-0232,
Pager 812-481-0897
Confidential information, Authorized use only.
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