I would suggest you check the lot#. You may have received a bad lot and need to
get them replaced by the vendor.
>>
> Hi all,
>
> We have suddenly started losing tissue sections from our Superfrost Plus
> Slides.
> Our students have been cutting fixed, frozen, cryosections (20um) and
> thaw-mounting these onto Superfrost Plus slides. We have suddenly started
> losing lots of sections from these slides (again!!). The tissue is small -
> ie cross sections of frog aorta and longitudinal sections of nerves, so any
> lose of adhesion results in total loss of the tissue. The odd thing is that
> she is not losing every section on every slide, but half to 3/4 of the
> sections are falling off within the first rinse. The sections are from the
> same tissue block on the same slide while some falls and others don't.
>
> We are at a lose as to what we can do to rescue these sections.
>
> Does anyone know if there is any way to coat the slides in some solution with
> the tissue on them to help improve the adhesion without losing the ability to
> do immunofluorescence?
>
> Any further advice on cutting / drying protocols are welcomed.
> This keeps happening and the inconsistency of it has us so frustrated with
> this that we are thinking of going back to subbing our own slides.
>
> Thanks for your help!
>
> Angelina
>
>
> -- ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o
>
> Angelina Y. Fong, Ph.D.
> Department of Zoology
> Biological Sciences Building
> 6270 University Boulevard
> University of British Columbia
> Vancouver, BC, V6T 1Z4
> Canada
> Ph: (604) 822-5799
> Fax: (604) 822-2416
> Email: f...@zoology.ubc.ca
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
