Yea if you were looking for fat that will be lost with paraffin processing, so the oil red o for fat will not work. I would not see any point in trying to depara and then snap freeze, but if I did want to do that since the tissue is formalin fixed I would depara, fix some more in formalin and then infiltrate the tissue in 30% sucrose overnight before snap freezing, if you do not do that fix tissue will not section well frozen.
Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pru...@ihctech.net -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, November 13, 2012 10:15 AM To: z o n k e d Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Help! Liver mistakenly processed in paraffin (had tobe in OCT instead)! It depends on what you are using the oil red o for. Lipofuscin and ceroid can be demonstrated with an oil red o stain after processing. Jennifer MacDonald From: z o n k e d <zon...@gmail.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: 11/13/2012 08:53 AM Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Sent by: histonet-boun...@lists.utsouthwestern.edu Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- "It costs nothing to say something kind. Even less to shut up altogether." --Nathan Fillion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet