RE: [Histonet] Immuno's
Who are you purchasing your antibodies from? Any of the companies that I have dealt with would gladly send someone out to optimize their antibodies. The fact that you are performing them manually probably is not the cause for the weak staining. We used to use the old Shandon Sequenza staining system as a backup if our Dako was out of service. When used with the same protocols, the staining was the same. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Hannen, Valerie Sent: Monday, April 11, 2011 10:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno's Hi Folks.. Looking for a bit of help. Is there anyone out there who are doing their immuno's manually? Our Pathologist has just informed us that our immuno's are ALL too weak. He states that they always have been, as he compares them to slides that we get back from the reference labs that we use for the antibodies that we don't do in-house. If you are doing them manually...what companies are you using for antibodies, detection kit and chromogen? And are your stains really dark? I informed our Pathologist that I know for a fact that the reference labs that we use, do the immuno's on stainers, and naturally these stains will be consistently be stronger. I am trying to see if I can keep costs down by trying not to have to buy a stainer and continue to do the immuno's manually. If you all know of a wet-workshop or of a immuno. company who would send someone to our facility to teach us ( the latter is preferred, since my section chief is going to retire in 1 1/2 weeks, I will be taking over her duties and this is going to leave us with only myself and one other tech, until we can get a replacement for my position). Any help or advice that can be given will be greatly appreciated. Thanks so much. Valerie Hannen, MLT(ASCP),HTL,SU (FL) Parrish Medical Center Titusville,Florida ** This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Immuno's
Toni beat me to it. My suggestion is to contact the vendor where you purchase your detection. The technical support departments for immuno vendors are emmensely helpful. Try Dako at 800-400-DAKO, Biocare at 603-925-8000, or Cellmarque at 800.665.7284. In addition, it has always been my experience that a good hand stain is always better than an instrument stain -- primarily because they are better washed by hand. My best guess is that you are not draining your slides well enough between rinses. What I do is flood my slides with buffer, then put them at an angle (the Biocare IQ stainer is great for this) and rinse again -- Robin Simpkins at Biocare calls this the Jamaican waterfall. I then follow up with a flick of the slide, then proceed to the next step. Again call your vendors. Technical support will be extremely helpful and you may get an expert (applications specialist or TSR) out to help you in person. William Chappell, HTL(ASCP)QIHC IHC Development Specialist CellNetix Pathology Laboratories (503) 358-9567 www.cellnetix.com Our expertise...your peace of mind --- On Mon, 4/11/11, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: From: Rathborne, Toni trathbo...@somerset-healthcare.com Subject: RE: [Histonet] Immuno's To: Hannen, Valerie valerie.han...@parrishmed.com, histonet@lists.utsouthwestern.edu Date: Monday, April 11, 2011, 8:38 AM Who are you purchasing your antibodies from? Any of the companies that I have dealt with would gladly send someone out to optimize their antibodies. The fact that you are performing them manually probably is not the cause for the weak staining. We used to use the old Shandon Sequenza staining system as a backup if our Dako was out of service. When used with the same protocols, the staining was the same. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]On Behalf Of Hannen, Valerie Sent: Monday, April 11, 2011 10:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno's Hi Folks.. Looking for a bit of help. Is there anyone out there who are doing their immuno's manually? Our Pathologist has just informed us that our immuno's are ALL too weak. He states that they always have been, as he compares them to slides that we get back from the reference labs that we use for the antibodies that we don't do in-house. If you are doing them manually...what companies are you using for antibodies, detection kit and chromogen? And are your stains really dark? I informed our Pathologist that I know for a fact that the reference labs that we use, do the immuno's on stainers, and naturally these stains will be consistently be stronger. I am trying to see if I can keep costs down by trying not to have to buy a stainer and continue to do the immuno's manually. If you all know of a wet-workshop or of a immuno. company who would send someone to our facility to teach us ( the latter is preferred, since my section chief is going to retire in 1 1/2 weeks, I will be taking over her duties and this is going to leave us with only myself and one other tech, until we can get a replacement for my position). Any help or advice that can be given will be greatly appreciated. Thanks so much. Valerie Hannen, MLT(ASCP),HTL,SU (FL) Parrish Medical Center Titusville,Florida ** This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset
RE: [Histonet] Immuno's
Do you use a hydrophobic pen to circle the tissue. This concentrates the solution where you need it. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 etx.232 e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, April 11, 2011 10:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno's Hi Folks.. Looking for a bit of help. Is there anyone out there who are doing their immuno's manually? Our Pathologist has just informed us that our immuno's are ALL too weak. He states that they always have been, as he compares them to slides that we get back from the reference labs that we use for the antibodies that we don't do in-house. If you are doing them manually...what companies are you using for antibodies, detection kit and chromogen? And are your stains really dark? I informed our Pathologist that I know for a fact that the reference labs that we use, do the immuno's on stainers, and naturally these stains will be consistently be stronger. I am trying to see if I can keep costs down by trying not to have to buy a stainer and continue to do the immuno's manually. If you all know of a wet-workshop or of a immuno. company who would send someone to our facility to teach us ( the latter is preferred, since my section chief is going to retire in 1 1/2 weeks, I will be taking over her duties and this is going to leave us with only myself and one other tech, until we can get a replacement for my position). Any help or advice that can be given will be greatly appreciated. Thanks so much. Valerie Hannen, MLT(ASCP),HTL,SU (FL) Parrish Medical Center Titusville,Florida ** This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
