Re: [Histonet] Isopentane storage-the polling is open
Melanie, Who's your vendor for buying Novec 7000? Maria Mejia San Francisco, CA Affymetrix On Apr 17, 2013, at 10:08 AM, mwh...@mcleodhealth.org wrote: I just wanted to clarify that we don't use Novec 7000 for freezing of muscle tissue. We use it for other routine frozen sections on humans.Sorry if that caused any confusion. Thanks Tim for pointing that out! Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmas...@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Isopentane storage-the polling is open
For almost two years, we have been using- with great success- a much safer alternative to Isopentane. It is called Novec 7000 from 3M. I am NOT a paid endorser and these are my views only , and do not reflect in any way on my employer. Our pathologists greatly prefer the results of a freezing bath over the cryostat peltier method. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmas...@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Isopentane storage-the polling is open
Always in an explosion proof refrigerator for storage. The isopentane in use is in an isopentane tank (at -70C) in the frozen section area of the gross room as is the fridge Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Garrison, Becky Sent: Tuesday, April 16, 2013 2:17 PM To: 'Paula Sicurello'; HistoNet Subject: RE: [Histonet] Isopentane storage-the polling is open We store at room temperature in a flammable cabinet. Use it to freeze muscle biopsies. The Isopentane is pre-cooled by immersing a container of isopentane in a container of Liquid Nitrogen. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 16, 2013 1:35 PM To: HistoNet Subject: [Histonet] Isopentane storage-the polling is open Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Isopentane storage-the polling is open
I just wanted to clarify that we don't use Novec 7000 for freezing of muscle tissue. We use it for other routine frozen sections on humans.Sorry if that caused any confusion. Thanks Tim for pointing that out! Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmas...@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Isopentane storage-the polling is open
We store our open bottle in an explosion proof fridge and unopened at room temp in a flammable storage cabinet Julia Celebre Sr MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 16, 2013 1:35 PM To: HistoNet Subject: [Histonet] Isopentane storage-the polling is open Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Isopentane storage-the polling is open
We maintain an approximately 1 liter supply of isopentane and store all isopentane (open/unopened) in an explosion proof freezer. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 16, 2013 12:35 PM To: HistoNet Subject: [Histonet] Isopentane storage-the polling is open Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Isopentane storage-the polling is open
Hi Julia, Thanks for the information. Can you tell me why you store it in the refrigerator? Paula Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 On Tue, Apr 16, 2013 at 1:39 PM, Celebre Julia celeb...@hhsc.ca wrote: We store our open bottle in an explosion proof fridge and unopened at room temp in a flammable storage cabinet Julia Celebre Sr MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 16, 2013 1:35 PM To: HistoNet Subject: [Histonet] Isopentane storage-the polling is open Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Isopentane storage-the polling is open
Definitely make it explosion-proof. See the following article by Robert Skinner in Sakura's Histo-Logic. http://www.sakura-americas.com/histologic/pdf/03_may.pdf He had an explosion in a freezer, when a researcher tried to store 50 mL (about $2.00) worth of isopentane in a regular freezer, and the lid on the container wasn't on tight enough. Fortunately, it was the middle of the night, and no one was in the room. The freezer door flew 12 feet, and there was lots of flames. See the photos! The article mentions the damage at $165,000, but I talked with Robert about a year later, and he said the total was over $1,000,000 when everything was factored in. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Health System Royal Oak, MI 48073 The opinions expressed are mine, and do not reflect on my place of employment. -Original Message- From: Morken, Timothy Sent: Tuesday, April 16, 2013 2:32 PM To: Paula Sicurello ; HistoNet Subject: RE: [Histonet] Isopentane storage-the polling is open Paula, we keep ours at room temp in a small flam cab near the muscle bench. We keep two gallons on hand. In Dapson Dapson's book Hazardous Materials in the Histopathology Laboratory (Anatech, 2005) they do not make any specific recommendations concerning storage temperature, except to say IF it is kept in the refrigerator/freezer use only an explosion-proof refrigerator/freezer. The flash point is -50C, so a fridge is not significantly different than room temp, unless your lab is often over 30C/85F (the boiling point!). Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 16, 2013 10:35 AM To: HistoNet Subject: [Histonet] Isopentane storage-the polling is open Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
