Thanks for your information Jack. I will look into the Acrylosin as an option
for doing MMA samples.
We are using the LR White as an alternative to GMA. We have had lots of trouble
with getting it to polymerize evenly and consistently. So now, of course, we
are having issues with knife lines and wrinkling with this resin.
I would be interested in getting your images if you would please send them to
me directly. I believe the list serve blocks them.
Best wishes,
Teri
From: Jack Ratliff [mailto:ratliffj...@hotmail.com]
Sent: Wednesday, July 27, 2011 4:56 PM
To: Johnson, Teri; Histonet
Subject: RE: [Histonet] LR White
Teri,
You should try using Haupt's Adhesive
(www.dornandhart.comhttp://www.dornandhart.com/) to secure your resin
sections. I have never (knocking twice on wood...LOL) lost a section during the
staining of thin resin sections when using this product. In fact, I have used
this solution for over 14 years and I even subject my methacrylate based resin
(Acrylosin SOFT Embedding Solution @
www.dornandhart.comhttp://www.dornandhart.com/) to deplastification with
warmed xylenes prior to staining and still do not lose a section. Also, I have
found that wrinkle free is proportional to the softness of the resin block
and the section collection, transfer and mounting method. I have attached a few
images of specimens embedded with Acrylosin and stained HE, Goldner's
Trichrome, and Von Kossa - MacNeal's Tetrachrome for your consideration. Feel
free to contact me if you have any additional questions.
On a related note, I am giving a teleconference sponsored by the National
Society for Histotechnology (NSH) next month (August 17th) as part of their VIR
Summer Teleconference Series. During this teleconference I will be talking
about the use of resin for undemineralized bone histology. Definitely check
this out if you have interest in working with undemineralized bone!
Resin Histology: A Practical Approach for Demonstrating Undemineralized Bone
Presented by Jack Ratliff, BioMimetic Therapeutics, Inc.
As musculoskeletal research progresses with new technological advancements in
the areas of biological repair and replacement, histological evaluation
continues to play a crucial role in the determination of safety and efficacy
for these new treatments. While most will employ traditional and acceptable
methods of decalcification and paraffin embedding for the demonstration of
these critical components of evaluation, these techniques can sometimes be very
challenging and/or impossible when presented with a variety of implant
materials or devices. For example, to evaluate safety and efficacy of a
metallic device coated with a biological therapeutic at the bone interface, one
will need to forego traditional methods of decalcification and seek an
undisturbed representation of the specimen by utilizing an embedding media that
is both as hard as the specimen and the implant material. Additionally, it may
also be important to use a media that will not distort or dissolve the coating.
This seminar will address the use of resin histology techniques for the
demonstration of undemineralized bone. Topics will include tissue preparation,
fixation, processing, infiltration, and embedding/polymerization with acrylic
resins. We will also discuss two types of microtomy as related to small and
large undemineralized bone specimens and the presence or absence of implant
materials.
Best Regards,
Jack
Jack L Ratliff
Senior Histologist
BioMimetic Therapeutics, Inc.
Chairman, Hard Tissue Committee
National Society for Histotechnology
From: t...@stowers.org
To: histonet@lists.utsouthwestern.edu
Date: Tue, 26 Jul 2011 10:29:06 -0500
Subject: [Histonet] LR White
Is anyone out there using LR White for routine resin embedding, sectioning,
and staining? I am interested in learning some tips for mounting sections on
to the slide as wrinkle free as possible. Also our HE stains are a little
bit pale.
Thanks in advance!
Teri
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