Hi, SMAD4 is very difficult on the Ventana platform. The only clone that would work on the Ventana to my knowledge(clone EP618Y, immunologic) has recently 'collapsed' (i hope that's the right word?) It means that it is not available anymore. Maybe there is some vendor that has a few vials left, but my supplier does not. If anybody knows of some other clone for the Ventana, I am very interested. MDM2: our sarcoma pathologist says that the MDM2 immuno is not reliable. Some liposarcomas will be negative. It would be better to do FISH, which is also very difficult because the fatty tissue often rinses off the slides. Willem
________________________________ Van: histonet-boun...@lists.utsouthwestern.edu namens Sue Hunter Verzonden: do 5-12-2013 16:50 Aan: Histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] M2D2 and SMAD Good morning Does anyone have suggestions for antibodies for M2D2 and SMAD? We have not been able to get these antibodies to work for us on the Ventana Ultras. If you do have suggestions, protocols and control tissue suggestions would also be appreciated. Thanks Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu<mailto:shun...@beaumont.edu> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet