Re: [Histonet] Negative Controls in IHC

[Rene J Buesa]

Negative controls for IHC has been discussed before many times but it keeps 
pupping up every noew and then.
This is my take on it:
1- if CAP requires them, so then  do it. You do not want to have a negative 
issue in your inspection
2- it is always good to have them for quality control of your procedure and to 
prevent any legal issues down the road
3- if I have a case requiring several antibodies, I only run a negative control 
for the tissue series (the block in question) but not for every antibody but if 
in that antibodies series there are different detection systems, I run a 
negative control for each
René J.



From: Ann Specian thisis...@aol.com
To: histonet@lists.utsouthwestern.edu 
Sent: Saturday, September 29, 2012 1:56 PM
Subject: [Histonet] Negative Controls in IHC


I have a question in regard to eliminating the use of negative controls when 
using a non-avidin-biotin detection system.

Do you not feel that negative controls may still need to be run in tissues 
which are likely to be pigmented such as lymph node, skin and liver?

We were thinking to eliminate the use of negatives for other tissues (cervical, 
GI, etc.) only.What is the general consensus?
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RE: [Histonet] Negative Controls in IHC

[Weems, Joyce K.]

We have eliminated them all per the CAP guidelines.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian
Sent: Saturday, September 29, 2012 1:56 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Negative Controls in IHC


I have a question in regard to eliminating the use of negative controls when 
using a non-avidin-biotin detection system.

Do you not feel that negative controls may still need to be run in tissues 
which are likely to be pigmented such as lymph node, skin and liver?

We were thinking to eliminate the use of negatives for other tissues (cervical, 
GI, etc.) only.What is the general consensus?
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