Re: [Histonet] Ada's Mail Stain line order
Hi Anne, Are they using the term "clearing" loosely, but really mean "rinsing"? The term "clearing" typically refers to the step after the alcohol (dehydration) and before coverslipping (if using a permanent, solvent based mounting media). Not sure why someone would "clear" before the rinse and bluing step in frozen section staining. For paraffin embedded specimens, the slide would need to Deparaffinize (in clearing agent-xylene or xylene substitute), dehydrate (graded alcohols—high to low), water rinse (preferably DI), stain the nuclei (hematoxylin), water rinse, decolorize (acid alcohol-if staining regressively), water rinse(s), bluing, water rinses, graded alcohol–low to high, cytoplasmic staining (Eosin), alcohol rises, clearing. For frozen section: 100% alcohol (or other fixative), water rise, hematoxylin (usually progressive, so no need to decolorize), bluing, water rinse, etc… You may choose to skip bluing as tap water can do it. Although for consistency it should be kept in (tap water pH can with seasons, water treatment facilty processes, etc.) Dee Wolfe On Oct 26, 2021, at 12:26 PM, Anne Murvosh via Histonet wrote: > When doing frozen section staining for Mohs we have DI water and don't need a > clearing agent. I am helping set up someone who is using tap water and needs > to clear it. They mentioned that they used to clear first, rinse, then use > bluing. I thought it was the opposite after heme. Rinse, bluing, rinse, > clearing. I don't remember which is first. Actually do you need a bluing if > using tap water since that blues better then DI water? Thanks for your input, > it's been 10 years since I had to think about this. Anne > > > Anne Murvosh > Histology Technician > > > > This electronic transmission and any documents accompanying this electronic > transmission may contain information that is confidential and/or legally > privileged. The information is intended only for the use of the individual or > entity named above. If you are not the intended recipient, you are hereby > notified that any disclosure, copying, distribution or the taking of any > action in reliance on or regarding the contents of this electronically > transmitted information is strictly prohibited. If you have received this > e-mail in error, please notify the sender and delete this message immediately. > > HIPAA Confidentiality Notice: The information and documents accompanying this > e-mail may contain confidential information that is legally privileged and > protected by federal and state law. The information is intended for use only > by the entity or individual to whom it is addressed, the authorized > recipient. The authorized recipient is obligated to maintain the information > in a safe, secure, and confidential manner. The authorized recipient is > prohibited from using the information for purposes other than intended, > prohibited from disclosing the information to any other party unless required > to do so by law or regulation, and is required to destroy the information > after its stated need has been fulfilled. If you are in possession of this > information, and are not the intended recipient, you are hereby notified that > any improper disclosure, copying, or distribution of the information is > strictly prohibited. Please notify the owner of the information immediately > and arrange for its return > or destruction. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ada's Mail Re: looking for cassettes
Hi LeRoy, Check out the Cancer Diagnostics website. Not sure what size you are looking for but they carry a similar cassette. https://www.cancerdiagnostics.com/consumables/cassettes Dee Dee Wolfe Anatech Ltd. | VP Technical Service & Manufacturing |1020 Harts Lake Road | Battle Creek, MI 49037 USA | phone +1.800.262.8324 ext 111 | fax +1.269.964.8084 | deewo...@anatechltdusa.com | www.anatechltdusa.com On Sep 9, 2021, at 4:36 PM, LEROY H BROWN via Histonet wrote: > HI. Anybody have a supplier for Klinipath cassettes? I am having a very > difficult time finding them these days. > > Thanks > > LeRoy Brown HT(ASCP) HTL > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ada's Mail Retirement in sight!
Congratulations, Tim…and what a story! Enjoyed "working with you" on the Michigan Society website when Thermo sponsored state society sites! Dee Dee Wolfe MSH Immediate Past President On Sep 9, 2021, at 12:26 PM, Morken, Timothy via Histonet wrote: > > After 40 years in the lab I've decided to retire this year - in a week > actually! > > It has been an interesting 4 decades... > > I started out in an EM lab after getting a degree in Physiology and then > competing a 2 year EM course at Delta College in Stockton, CA - the only > dedicated EM program at that time. I started out running a scanning EM lab > for an electronics company looking at microchips but after a couple years > moved to a hosptial lab in Fresno, CA running their EM lab. I was the only > one, so from day one was the "Manager" of the lab! I did about 150 EM cases a > year and in those days it was a mix of kidney and tumor cases - there was no > IHC yet so some tumor diagnostics depended on EM. I did not have quite enough > work to keep me busy so I started hanging out in the histology lab. As with > many people in this field the day I started working there was the first I had > heard of "histology." At first it was helping set up grossing, coverslipping > slides and doing immunofluorescence for the kidney cases (and taking > "kodachromes" of the results! Does anyone under 30 know what a Kodachrome > is?!). But then our > director wanted to bring in IHC and so had a tech from a lab at Cedars Sinai > in LA come to teach us how to do it. We did all of 10 stains at first. Of > course it was all manual and so had to know what was going on with every > step. I didn't use an automated stainer for the first 12 years that I did > IHC, and at times was doing 150 slides a day manually. > > Gradually I ended up doing half time in histology and learned cutting, > special stains, muscle histochemistry, immunofluorescence for kidney cases. I > decided to work on the HT exam since I was doing all that work anyway. We had > a lab of four men - pretty rare, Imagine - and we started a study group to > all take the test. We met after work a couple times a week for 6 months > pretty much memorizing the Sheehan book. We all took the HT and all but one > passed. Later I passed the HTL as well. > > After 11 years of that I moved on to a job in Saudi Arabia - and my wife and > daughter went along. I managed the IHC and muscle lab at King Faisal > Specialist Hospital in Riyadh. My wife was lucky enough to get a teaching > position at the American School where our daughter was in 9th grade. That > made all the difference in our life there because if she had not gotten a job > I don't think we would have stayed there 5 years. She would have been stuck > doing pretty much nothing. I moved on to managing the histology lab as > whole. Living in another country is a great experience, even if it is a > totally different culture. It certainly changed our outlook on the world and > I would not trade that experience for anything. We also did a lot of > travelling during those years - being on "that" side of world makes > traveling there much easier! > > Once we decided to leave Saudi I looked for a job back in the States and was > lucky enough to land one at the Centers for Disease Control in Atlanta in > their Infectious Disease Pathology division. I worked with 5 infectious > disease pathology specialists and a dozen technologists from histotechs to EM > techs, to microbiologists to molecular biologists. We worked on routine cases > to world-wide outbreak cases. During the 5 years I was there we identified at > least one novel human virus every year that caused outbreaks. And that was in > addition to numerous cases of outbreaks of known diseases for which we > received samples from all over the world. Probably the most notorious case > was the anthrax attack after 9/11. Four of us histotechs manned the lab 24 > hours a day, 7 days a week for 6 weeks running IHC tests on endless samples > while trying to get on top of that case. In the middle of it all the power > went out to the facility and we had to work on generator power with temporary > lighting set > up in the lab and battery packs to keep the equipment running. After 9/11 and > then anthrax everyone was thinking it was a bioterror attack by the same > group, so things were crazy. When I think of all the efforts we made to > enhance our detection and diagnostic capabilities, and all our meetings about > how to handle outbreaks, it was hard to see the stumbles the CDC made in this > current pandemic. But I can say that we had discussed, studied and predicted > pretty much everything that has happened in this Covid 19 era. Indeed, we had > the first-hand experience with SARS in the last year I was there, so knew > exactly how it could play out. > > Finally we decided to move back to California and I was able to connect with > an
RE: [Histonet] No Mail??
And I kept getting a returned msg yesterday that I was trying to post. I am experiencing withdrawal!!! :)j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, December 17, 2010 10:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] No Mail?? Any Problem? I have not received any email from Histo-net last few days ?? Behnaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] No Mail??
Wow...didn't realize how addicted to Histonet I'd become till this week! Sandy Harrison Histology Supervisor VA Medical Center Minneapolis, MN 55417 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 9:54 AM To: Behnaz Sohrab; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] No Mail?? And I kept getting a returned msg yesterday that I was trying to post. I am experiencing withdrawal!!! :)j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, December 17, 2010 10:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] No Mail?? Any Problem? I have not received any email from Histo-net last few days ?? Behnaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] No Mail??
Oh man! The first time Histonet stopped working, I thought it was my email... but this time I knew better! And I too didn't realize how much I looked forward to the messages/thoughts/questions shared on this board. So histonet, what's been causing the problem? Michelle On Dec 17, 2010, at 11:29 AM, Harrison, Sandra C. sandra.harris...@va.gov wrote: Wow...didn't realize how addicted to Histonet I'd become till this week! Sandy Harrison Histology Supervisor VA Medical Center Minneapolis, MN 55417 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, December 17, 2010 9:54 AM To: Behnaz Sohrab; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] No Mail?? And I kept getting a returned msg yesterday that I was trying to post. I am experiencing withdrawal!!! :)j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, December 17, 2010 10:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] No Mail?? Any Problem? I have not received any email from Histo-net last few days ?? Behnaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
