Re: [Histonet] RE: Cornflaking artifact
We had this issue when we were using the tape from Mercedes Medical and went back to Sakura tape and it went away. It could also be associated with humidity. STP TJUH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Cornflaking artifact
Long time and high volume user of the Sakura Tape. Check the Xylene drops. We coverslip thousands of slides daily and have seen no isssue with the tape lots. Our experience shows some cornflaking when the slide does not receive enough xylens to wet the slide properly. William DeSalvo, BS HTL(ASCP) > From: kenneth.metz...@aruplab.com > To: sheila.herring...@interiorhealth.ca; lcolb...@pathmdlabs.com; > sscal...@beaumont.edu; histonet@lists.utsouthwestern.edu > Date: Thu, 13 Mar 2014 16:13:24 + > CC: > Subject: [Histonet] RE: Cornflaking artifact > > Can anyone share the lot # on their coverslipping film (Sakura)? We are > seeing this as well out of the blue..haven't changed a thing. > > Kenneth G Metzger HTL(ASCP) > Histology Supervisor > ARUP Labs > Salt Lake City, Utah > Phone: (801)583-2787 ext. 3101 > Fax: (801) 584-5244 > Email: kenneth.metz...@aruplab.com > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of HERRINGTON, > SHEILA > Sent: Thursday, March 13, 2014 9:42 AM > To: 'Laurie Colbert'; Sharon Scalise; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Cornflaking artifact > > We also have recently started to see this artifact more than ever before, and > nothing in our process has changed. We have tried everything to correct to > no avail. Wonder if it is possible to be a change in some type of supply, > either xylene or coverslipping film. Something has changed but am at a loss > as to what. > > > Sheila Herrington > Technical Lead Histopathology and Immunohistochemistry Kelowna General > Hospital > 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 > 250-862-4300 ext 7587 or 7510 > sheila.herring...@interiorhealth.ca > > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert > Sent: Thursday, March 13, 2014 6:30 AM > To: Sharon Scalise; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Cornflaking artifact > > You will also see the cornflaking if your tissue is lifting off of the slide > at all. We used to get this more often on hard, decal specimens than on > other specimens. We used the film to coverslip. If you remove the film from > the problem slides and recoverslip conventionally with extra mountant and > glass coverslips, I'm sure you will not see the artifact. > > Laurie Colbert > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise > Sent: Wednesday, March 12, 2014 8:00 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Cornflaking artifact > > I am looking for help with "cornflaking" (tiny, brown dry spots under > coverslip)artifact. We have been using fresh xylene on our stainer and > coverslipper, cleaned and wiped all containers dry before filling, tried > different lots of coverslipping film and had service on our coverslipper to > make sure it was functioning properly, including the xylene drip. We > continue to have this artifact and it is driving us crazy. It is sporadic > with no pattern of tissue type or placement on the slide. Sometimes it lands > on tissue other times not. Most of the time when we remove the coverslip and > re-coverslip it goes away (I am assuming because the acetone removes any > minute amounts of water that may be present). We just cannot figure out > where the water is coming from. Has anyone seen this artifact while using > the drying step on the prisma stainer? We just recently started using the > drying on some slides and I am thinking maybe it is causing humidity??? I > cannot say for a fact that our "cornflaking" started at the same time, but it > is suspicious. HELP! > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor > Jordan > Sent: Wednesday, March 12, 2014 3:57 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Paraffin type and Tetracycline labelling Questions > > For those who have done Decalcified bone processing with paraffinwhat is > the best type of paraffin that you guys are familiar with? > > Also, if you are wanting to see a tetracycline label on the bone for bone > turnover, must undecalcified sections be used? How for a double tetracycline > label? > > > Trevor Jordan Wait > University of Texas Health Science Center, San Antonio Class of 2017 MD > Candidate Abilene Christian University Class of 2013 Graduate B.S. > Biochemistry ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouth
Re: [Histonet] RE: Cornflaking artifact
We had this problem several years ago. We were using the sakura tapes with the coverslipper. We did the following: Last three alcohols were changes frequently. Slides should be not dry when loading on coverslipper. If you could load two racks at a time, only load one. By this way the slides in the second rack will not dry out. Finally, change the tapes from sakura to Mercedes Medical tapes. Mala Nirmala Srishan Holy Name Medical Center From: "HERRINGTON, SHEILA" To: 'Laurie Colbert' , Sharon Scalise , "histonet@lists.utsouthwestern.edu" Date: 03/13/2014 11:43 AM Subject:[Histonet] RE: Cornflaking artifact Sent by:histonet-boun...@lists.utsouthwestern.edu We also have recently started to see this artifact more than ever before, and nothing in our process has changed. We have tried everything to correct to no avail. Wonder if it is possible to be a change in some type of supply, either xylene or coverslipping film. Something has changed but am at a loss as to what. Sheila Herrington Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 sheila.herring...@interiorhealth.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, March 13, 2014 6:30 AM To: Sharon Scalise; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact You will also see the cornflaking if your tissue is lifting off of the slide at all. We used to get this more often on hard, decal specimens than on other specimens. We used the film to coverslip. If you remove the film from the problem slides and recoverslip conventionally with extra mountant and glass coverslips, I'm sure you will not see the artifact. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise Sent: Wednesday, March 12, 2014 8:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cornflaking artifact I am looking for help with "cornflaking" (tiny, brown dry spots under coverslip)artifact. We have been using fresh xylene on our stainer and coverslipper, cleaned and wiped all containers dry before filling, tried different lots of coverslipping film and had service on our coverslipper to make sure it was functioning properly, including the xylene drip. We continue to have this artifact and it is driving us crazy. It is sporadic with no pattern of tissue type or placement on the slide. Sometimes it lands on tissue other times not. Most of the time when we remove the coverslip and re-coverslip it goes away (I am assuming because the acetone removes any minute amounts of water that may be present). We just cannot figure out where the water is coming from. Has anyone seen this artifact while using the drying step on the prisma stainer? We just recently started using the drying on some slides and I am thinking maybe it is causing humidity??? I cannot say for a fact that our "cornflaking" started at the same time, but it is suspicious. HELP! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor Jordan Sent: Wednesday, March 12, 2014 3:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin type and Tetracycline labelling Questions For those who have done Decalcified bone processing with paraffinwhat is the best type of paraffin that you guys are familiar with? Also, if you are wanting to see a tetracycline label on the bone for bone turnover, must undecalcified sections be used? How for a double tetracycline label? Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only
Re: [Histonet] RE: Cornflaking artifact
Is this a tape coverslipper? If so, you do have minute traces of water carryover to your xylene. If there is even the hint of pink in you last dehydrant before xylene, you will get that cornflake artifact. Acetone wont help, because water still be present. Increase number of absolute alcohol before xylene, and check often for eosin carryover. This fixed our problem. Jackie O' Sent from my iPhone > On Mar 12, 2014, at 21:59, Sharon Scalise wrote: > > I am looking for help with "cornflaking" (tiny, brown dry spots under > coverslip)artifact. We have been using fresh xylene on our stainer and > coverslipper, cleaned and wiped all containers dry before filling, tried > different lots of coverslipping film and had service on our coverslipper to > make sure it was functioning properly, including the xylene drip. We > continue to have this artifact and it is driving us crazy. It is sporadic > with no pattern of tissue type or placement on the slide. Sometimes it lands > on tissue other times not. Most of the time when we remove the coverslip and > re-coverslip it goes away (I am assuming because the acetone removes any > minute amounts of water that may be present). We just cannot figure out > where the water is coming from. Has anyone seen this artifact while using > the drying step on the prisma stainer? We just recently started using the > drying on some slides and I am thinking maybe it is causing humidity??? I > cannot say for a fact that our "cornflaking" started at the same time, but it > is suspicious. HELP! > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor > Jordan > Sent: Wednesday, March 12, 2014 3:57 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Paraffin type and Tetracycline labelling Questions > > For those who have done Decalcified bone processing with paraffinwhat is > the best type of paraffin that you guys are familiar with? > > Also, if you are wanting to see a tetracycline label on the bone for bone > turnover, must undecalcified sections be used? How for a double tetracycline > label? > > > Trevor Jordan Wait > University of Texas Health Science Center, San Antonio Class of 2017 MD > Candidate Abilene Christian University Class of 2013 Graduate B.S. > Biochemistry ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
