Classification: UNCLASSIFIED Caveats: NONE I have 2 Leica ASP 300 tissue processors. I use Thermo Scientific Shandon Xylene Substitute (formally known as Histosolv). I have used this for many years with excellent results. I have never had any problems using this in the processor. We had 2 very old VIP tissue processors before we got the Leica processors and used Histosolv in those processors also. I have never heard of Sub-X.
Diane C. Gladney, HT (ASCP) Supervisor, Anatomical Pathology Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Email: diane.glad...@amedd.army.mil Phone: 803-751- 2530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Wednesday, August 05, 2009 4:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Xylene substitutes William (Bill) O'Donnell, HT (ASCP) QIHC, Lead Histologist, Good Samaritan Hospital, Kearney, Nebraska, notes that Clear-Rite 3 is at least temporarily unavailable, and he is looking for a substitute. (Anybody know what's going on with Clear-Rite 3?) Clear-Rite 3 is an aliphatic (rather than a limonene). Aliphatics can't always be substituted for each other - they are chemically different from each other - each has its own flammability characteristics (flash point) and (if you're recovering solvent using a spinning band still) its own distillation routine. If you change, you may want to stay with the product you change to. Your purchasing people need to be aware that they are not to change brands on you. I just worked in a lab that has a Leica ASP300S processor (which they like, by the way). The people there have been told that they may use only one particular aliphatic with it, one I hadn't heard of before: Sub-X aliphatic hydrocarbon isoparaffinic oil CAS 6472-48-9 DOT petroleum distillates, NOS, 3 class 3 flammable liquid Flash point 106 F (41 C) Anybody know if this is actually the case? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Classification: UNCLASSIFIED Caveats: NONE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet