RE: [Histonet] Sectioning on different microtomes
I'm on the same page as you Jenaine- we get blocks from all over so we are constantly rearranging the block holder. We also do have a histocollimeter to get the Microms back in line (it doesn't fit on the Leicas. Berncie Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Saturday, January 24, 2009 6:00 PM To: arvidsonkris...@yahoo.com; histonet Subject: RE: [Histonet] Sectioning on different microtomes I simply align my block holder to match up with the block. We get many outside blocks so I have learned to be pretty good at this. From: histonet-boun...@lists.utsouthwestern.edu on behalf of kristen arvidson Sent: Sat 1/24/2009 7:11 AM To: histonet Subject: [Histonet] Sectioning on different microtomes How are people lining up the microtomes so they cut in approximately the plane. I use the old fashioned test paraffin block method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own. Our paths have complained about recuts being too deep. HELP!!! Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sectioning on different microtomes
There are microtome aligners that can help. They are available for Leica, Microm and Shandon Finesse. We used to use them here before we got all of the same microtomes. They worked pretty well. We purchased ours from Newcomer Supply. They're super easy to use and take lots less time than the paraffin block method. Hope this helps. Jodie Robertson, HT (ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Saturday, January 24, 2009 4:11 AM To: histonet Subject: [Histonet] Sectioning on different microtomes How are people lining up the microtomes so they cut in approximately the plane. I use the old fashioned test paraffin block method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own. Our paths have complained about recuts being too deep. HELP!!! Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sectioning on different microtomes
I don't know how these aligning tools work, but I always used an inexpensive level from the hardware store. You need one that can read in the vertical position. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Jodie Robertson wrote: There are microtome aligners that can help. They are available for Leica, Microm and Shandon Finesse. We used to use them here before we got all of the same microtomes. They worked pretty well. We purchased ours from Newcomer Supply. They're super easy to use and take lots less time than the paraffin block method. Hope this helps. Jodie Robertson, HT (ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Saturday, January 24, 2009 4:11 AM To: histonet Subject: [Histonet] Sectioning on different microtomes How are people lining up the microtomes so they cut in approximately the plane. I use the old fashioned test paraffin block method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own. Our paths have complained about recuts being too deep. HELP!!! Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sectioning on different microtomes
Meet with your techs and ask about which angle they prefer and why. Get to a consensus about a compromise angle they all can agree with and IMPLEMENT the angle. Put it in the SOP and check that everybody complies. René J. --- On Sat, 1/24/09, kristen arvidson arvidsonkris...@yahoo.com wrote: From: kristen arvidson arvidsonkris...@yahoo.com Subject: [Histonet] Sectioning on different microtomes To: histonet histonet@lists.utsouthwestern.edu Date: Saturday, January 24, 2009, 7:11 AM How are people lining up the microtomes so they cut in approximately the plane. I use the old fashioned test paraffin block method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own. Our paths have complained about recuts being too deep. HELP!!! Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sectioning on different microtomes
I simply align my block holder to match up with the block. We get many outside blocks so I have learned to be pretty good at this. From: histonet-boun...@lists.utsouthwestern.edu on behalf of kristen arvidson Sent: Sat 1/24/2009 7:11 AM To: histonet Subject: [Histonet] Sectioning on different microtomes How are people lining up the microtomes so they cut in approximately the plane. I use the old fashioned test paraffin block method but it just seems so time consuming and it doesn't always yield the best results especially if you are having techs line up there own. Our paths have complained about recuts being too deep. HELP!!! Kristen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
